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cc-by (c) Guirado, Pedro et al., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/159039

Differential distribution fo the wlaN and cgtB genes, associated with Guillain-Barré syndrome, in Campylobacter jejuni isolates from humans, broiler chickens, and wild birds

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Campylobacter jejuni causes campylobacteriosis, a bacterial gastroenteritis with high incidence worldwide. Moreover, C. jejuni infection can trigger the polyneuropathic disorder denominated Guillain-Barré syndrome (GBS). The C. jejuni strains that can elicit GBS carry either wlaN or cgtB, coding both genes for a β-1,3-galactosyltransferase enzyme that is required for the production of sialylated lipooligosaccharide (LOSSIAL). We described a differential prevalence of the genes wlaN and cgtB in C. jejuni isolates from three different ecological niches: humans, broiler chickens, and wild birds. The distribution of both genes, which is similar between broiler chicken and human isolates and distinct when compared to the wild bird isolates, suggests a host-dependent distribution. Moreover, the prevalence of the wlaN and cgtB genes seems to be restricted to some clonal complexes. Gene sequencing identified the presence of new variants of the G- homopolymeric tract within the wlaN gene. Furthermore, we detected two variants of a G rich region within the cgtBgene, suggesting that, similarly to wlaN, the G-tract in the cgtB gene mediates the phase variation control of cgtB expression. Caco-2 cell invasion assays indicate that there is no evident correlation between the production of LOSSIAL and the ability to invade eukaryotic cells.

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GUIRADO, Pedro, PAYTUBI CASABONA, Sònia, MIRÓ, Elisenda, IGLESIAS-TORRENS, Yaidelis, NAVARRO, Ferran, CERDÀ CUÉLLAR, Marta, STEPHAN-OTTO ATTOLINI, Camille, BALSALOBRE PARRA, Carlos, MADRID XUFRÉ, Cristina. Differential distribution fo the wlaN and cgtB genes, associated with Guillain-Barré syndrome, in Campylobacter jejuni isolates from humans, broiler chickens, and wild birds. _Microorganisms_. 2020. Vol. 8, núm. 325. [consulta: 20 de gener de 2026]. ISSN: 2076-2607. [Disponible a: https://hdl.handle.net/2445/159039]

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