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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/104175
Treatment with G-CSF reduces acute myeloid leukemia blast viability in the presence of bone marrow stroma
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BACKGROUND: The resulting clinical impact of the combined use of G-CSF with chemotherapy as a chemosensitizing strategy for treatment of acute myeloid leukemia (AML) patients is still controversial. In this study, the effect of ex vivo treatment with G-CSF on AML primary blasts was studied. METHODS: Peripheral blood mononuclear cells from AML patients were treated with G-CSF at increasing doses, alone or in co-culture with HS-5 stromal cells. Cell viability and surface phenotype was determined by flow cytometry 72 h after treatment. For clonogenicity assays, AML primary samples were treated for 18 h with G-CSF at increasing concentrations and cultured in methyl-cellulose for 14 days. Colonies were counted based on cellularity and morphology criteria. RESULTS: The presence of G-CSF reduced the overall viability of AML cells co-cultured with bone marrow stroma; whereas, in absence of stroma, a negligible effect was observed. Moreover, clonogenic capacity of AML cells was significantly reduced upon treatment with G-CSF. Interestingly, reduction in the AML clonogenic capacity correlated with the sensitivity to chemotherapy observed in vivo. CONCLUSIONS: These ex vivo results would provide a biological basis to data available from studies showing a clinical benefit with the use of G-CSF as a priming agent in patients with a chemosensitive AML and would support implementation of further studies exploring new strategies of chemotherapy priming in AML.
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NOMDEDEU I FÀBREGA, Meritxell, LARA CASTILLO, María carmen, ETXABE, Amaia, CORNET MASANA, Josep maria, PRATCORONA, Marta, DÍAZ BEYÀ, Marina, CALVO, Xavier, ROZMAN, María, COSTA, Dolors, ESTEVE REYNER, Jordi, RISUEÑO, Ruth m.. Treatment with G-CSF reduces acute myeloid leukemia blast viability in the presence of bone marrow stroma. _Cancer Cell International_. 2015. Vol. 15, núm. 122. [consulta: 20 de gener de 2026]. ISSN: 1475-2867. [Disponible a: https://hdl.handle.net/2445/104175]