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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/139019
High frequency of the exoU+/exoS+ genotype associated with multidrug-resistant "high-risk clones" of Pseudomonas aeruginosa clinical isolates from Peruvian hospitals
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The type III secretion system of Pseudomonas aeruginosa
is an important virulence factor contributing to the
cytotoxicity and the invasion process of this microorganism. The
current study aimed to determine the presence of the exoU+/exoS+
genotype in P. aeruginosa clinical isolates. The presence of
exoS, exoT, exoU and exoY was determined in 189 P. aeruginosa by
PCR, and the presence/absence of exoU was analysed according to
source infection, clonal relationships, biofilm formation,
motility and antimicrobial susceptibility. The gyrA, parC, oprD,
efflux pump regulators and \xCE\xB2-lactamases genes were also
analysed by PCR/sequencing. The exoS, exoT and exoY genes were
found in 100% of the isolates. Meanwhile, exoU was present in
43/189 (22.8%) of the isolates, being significantly associated
with multidrug resistance, extensively drug resistance as well
as with higher level quinolone resistance. However, the presence
of \xCE\xB2-lactamases, mutations in gyrA and parC, and relevant
modifications in efflux pumps and OprD were not significantly
associated with exoU+ isolates. MLST analysis of a subset of 25
isolates showed 8 different STs displaying the exoU+/exoS+
genotype. The MDR basis of the exoU+ isolates remain to be
elucidated. Furthermore, the clinical implications and spread of
exoU+/exoS+\xC2\xA0P. aeruginosa isolates need to be
established.
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HORNA, Gertrudis, et al. High frequency of the exoU+/exoS+ genotype associated with multidrug-resistant "high-risk clones" of Pseudomonas aeruginosa clinical isolates from Peruvian hospitals. Scientific Reports. 2019. Vol. 9. ISSN 2045-2322. [consulted: 7 of October of 2026]. Available at: https://hdl.handle.net/2445/139019