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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/189064
The trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling
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IL-6 has been shown to be required for somatic cell reprogramming into induced pluripotent stem cells (iPSCs). However, how Il6 expression is regulated and whether it plays a role during embryo development remains unknown. Here, we describe that IL-6 is necessary for C/EBPα-enhanced reprogramming of B cells into iPSCs but not for B cell to macrophage transdifferentiation. C/EBPα overexpression activates both Il6 and Il6ra genes in B cells and in PSCs. In embryo development, Cebpa is enriched in the trophectoderm of blastocysts together with Il6, while Il6ra is mostly expressed in the inner cell mass (ICM). In addition, Il6 expression in blastocysts requires Cebpa. Blastocysts secrete IL-6 and neutralization of the cytokine delays the morula to blastocyst transition. The observed requirement of C/EBPα-regulated IL-6 signaling for pluripotency during somatic cell reprogramming thus recapitulates a physiologic mechanism in which the trophectoderm acts as niche for the ICM through the secretion of IL-6.Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
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PLANA CARMONA, Marcos, STIK, Gregoire, BULTEAU, Romain, SEGURA MORALES, Carolina, ALCÁZAR, Noelia, WYATT, Cris d.r., KLONIZAKIS, Antonios, ANDRÉS AGUAYO, Luisa de, GASNIER, Maxime, TIAN, Tian v., TORCAL GARCIA, Guillem, VILA CASADESÚS, Maria, PLACHTA, Nicolas, SERRANO MARUGÁN, Manuel, FRANCESCONI, Mirko, GRAF, Thomas. The trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling. _Stem Cell Reports_. 2022. Vol. 17, núm. 9. [consulta: 9 de gener de 2026]. ISSN: 2213-6711. [Disponible a: https://hdl.handle.net/2445/189064]