The trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling

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dc.contributor.authorPlana Carmona, Marcos
dc.contributor.authorStik, Gregoire
dc.contributor.authorBulteau, Romain
dc.contributor.authorSegura Morales, Carolina
dc.contributor.authorAlcázar, Noelia
dc.contributor.authorWyatt, Cris D.R.
dc.contributor.authorKlonizakis, Antonios
dc.contributor.authorAndrés Aguayo, Luisa de
dc.contributor.authorGasnier, Maxime
dc.contributor.authorTian, Tian V.
dc.contributor.authorTorcal Garcia, Guillem
dc.contributor.authorVila Casadesús, Maria
dc.contributor.authorPlachta, Nicolas
dc.contributor.authorSerrano Marugán, Manuel
dc.contributor.authorFrancesconi, Mirko
dc.contributor.authorGraf, Thomas
dc.date.accessioned2022-09-16T06:23:36Z
dc.date.available2022-09-16T06:23:36Z
dc.date.issued2022-01-01
dc.date.updated2022-09-15T15:32:50Z
dc.description.abstractIL-6 has been shown to be required for somatic cell reprogramming into induced pluripotent stem cells (iPSCs). However, how Il6 expression is regulated and whether it plays a role during embryo development remains unknown. Here, we describe that IL-6 is necessary for C/EBPα-enhanced reprogramming of B cells into iPSCs but not for B cell to macrophage transdifferentiation. C/EBPα overexpression activates both Il6 and Il6ra genes in B cells and in PSCs. In embryo development, Cebpa is enriched in the trophectoderm of blastocysts together with Il6, while Il6ra is mostly expressed in the inner cell mass (ICM). In addition, Il6 expression in blastocysts requires Cebpa. Blastocysts secrete IL-6 and neutralization of the cytokine delays the morula to blastocyst transition. The observed requirement of C/EBPα-regulated IL-6 signaling for pluripotency during somatic cell reprogramming thus recapitulates a physiologic mechanism in which the trophectoderm acts as niche for the ICM through the secretion of IL-6.Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
dc.format.extent14 p.
dc.format.mimetypeapplication/pdf
dc.identifier.idimarina6565767
dc.identifier.issn2213-6711
dc.identifier.pmid35961310
dc.identifier.urihttps://hdl.handle.net/2445/189064
dc.language.isoeng
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1016/j.stemcr.2022.07.009
dc.relation.ispartofStem Cell Reports, 2022, vol. 17, num. 9
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/669622/EU//CELLPLASTICITY
dc.relation.urihttps://doi.org/10.1016/j.stemcr.2022.07.009
dc.rightscc by-nc-nd (c) Plana Carmona, Marcos et al., 2022
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.sourceArticles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
dc.subject.classificationEmbriologia
dc.subject.classificationFisiologia cel·lular
dc.subject.otherEmbryology
dc.subject.otherFisiologia cel·lular
dc.titleThe trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion

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