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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/193102
Serum Extracellular Vesicle-Derived microRNAs as Potential Biomarkers for Pleural Mesothelioma in a European Prospective Study
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Simple Summary Malignant pleural mesothelioma (MPM) is an aggressive and still incurable cancer. There is an urgent need to identify effective and reliable tools for detecting and diagnosing the early onset of MPM. In our study, we investigated the whole miRNAs expression profile from serum extracellular vesicles to identify early changes related to MPM development. miR-11400, miR-148a-3p, and miR-409-3p levels were increased in pre-clinical MPM patients up to five years before their diagnosis. The three-miRNA pattern showed a good discrimination capacity to distinguish pre-clinical MPM from cancer-free controls. The three miRNAs also displayed high diagnostic capabilities for differentiating between MPM patients and controls. This study identified a potential EV miRNA signature in preclinical MPM up to five years before diagnosis and raises the possibility of early intervention. Malignant pleural mesothelioma (MPM) is an aggressive cancer with a dismal prognosis. Early therapeutic interventions could improve patient outcomes. We aimed to identify a pattern of microRNAs (miRNAs) as potential early non-invasive markers of MPM. In a case-control study nested in the European Prospective Investigation into Cancer and Nutrition cohort, we screened the whole miRNome in serum extracellular vesicles (EVs) of preclinical MPM cases. In a subgroup of 20 preclinical samples collected five years prior MPM diagnosis, we observed an upregulation of miR-11400 (fold change (FC) = 2.6, adjusted p-value = 0.01), miR-148a-3p (FC = 1.5, p-value = 0.001), and miR-409-3p (FC = 1.5, p-value = 0.04) relative to matched controls. The 3-miRNA panel showed a good classification capacity with an area under the receiver operating characteristic curve (AUC) of 0.81 (specificity = 0.75, sensitivity = 0.70). The diagnostic ability of the model was also evaluated in an independent retrospective cohort, yielding a higher predictive power (AUC = 0.86). A signature of EV miRNA can be detected up to five years before MPM; moreover, the identified miRNAs could provide functional insights into the molecular changes related to the late carcinogenic process, preceding MPM development.
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CASALONE, Elisabetta, BIROLO, Giovanni, PARDINI, Barbara, ALLIONE, Alessandra, RUSSO, Alessia, CATALANO, Chiara, MENCOBONI, Manlio, FERRANTE, Daniela, MAGNANI, Corrado, SCULCO, Marika, DIANZANI, Irma, GROSSO, Federica, MIRABELLI, Dario, FILIBERTI, Rosa angela, RENA, Ottavio, SACERDOTE, Carlotta, RODRIGUEZ BARRANCO, Miguel, SMITH BYRNE, Karl, PANICO, Salvatore, AGNOLI, Claudia, JOHNSON, Theron, KAAKS, Rudolf, TUMINO, Rosario, HUERTA, José maría, RIBOLI, Elio, HEATH, Alicia k., TROBAJO SANMARTÍN, Camino, SCHULZE, Matthias b., SAIEVA, Calogero, AMIANO, Pilar, AGUDO, Antonio, WEIDERPASS, Elisabete, VINEIS, Paolo, MATULLO, Giuseppe. Serum Extracellular Vesicle-Derived microRNAs as Potential Biomarkers for Pleural Mesothelioma in a European Prospective Study. _Cancers_. 2022. Vol. 15, núm. 1, pàgs. 125. [consulta: 25 de gener de 2026]. ISSN: 2072-6694. [Disponible a: https://hdl.handle.net/2445/193102]