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2012-07-03

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cc-by (c) Amigó-Correig, Marta et al., 2012
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/114289

Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers

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https://doi.org/10.1371/journal.pone.0039087

Resum

Objective: This study aims at exploring the effects of sodium tungstate treatment on hypothalamic plasticity, which is known to have an important role in the control of energy metabolism. Methods: Adult lean and high-fat diet-induced obese mice were orally treated with sodium tungstate. Arcuate and paraventricular nuclei and lateral hypothalamus were separated and subjected to proteomic analysis by DIGE and mass spectrometry. Immunohistochemistry and in vivo magnetic resonance imaging were also performed. Results: Sodium tungstate treatment reduced body weight gain, food intake, and blood glucose and triglyceride levels. These effects were associated with transcriptional and functional changes in the hypothalamus. Proteomic analysis revealed that sodium tungstate modified the expression levels of proteins involved in cell morphology, axonal growth, and tissue remodeling, such as actin, CRMP2 and neurofilaments, and of proteins related to energy metabolism. Moreover, immunohistochemistry studies confirmed results for some targets and further revealed tungstate-dependent regulation of SNAP25 and HPC-1 proteins, suggesting an effect on synaptogenesis as well. Functional test for cell activity based on c-fos- positive cell counting also suggested that sodium tungstate modified hypothalamic basal activity. Finally, in vivo magnetic resonance imaging showed that tungstate treatment can affect neuronal organization in the hypothalamus. Conclusions: Altogether, these results suggest that sodium tungstate regulates proteins involved in axonal and glial plasticity. The fact that sodium tungstate could modulate hypothalamic plasticity and networks in adulthood makes it a possible and interesting therapeutic strategy not only for obesity management, but also for other neurodegenerative illnesses like Alzheimer's disease.

Matèries

Compostos de tungstè, Obesitat, Metabolisme, Diabetis, Ressonància magnètica, Expressió gènica, Malaltia d'Alzheimer, Rates (Animals de laboratori)

Matèries (anglès)

Tungsten compounds, Obesity, Metabolism, Diabetes, Magnetic resonance, Gene expression, Alzheimer's disease, Rats as laboratory animals

Citació

Col·leccions

Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Medicina)

Citació

AMIGÓ CORREIG, Marta, BARCELÓ BATLLORI, Sílvia, SORIA, Guadalupe, KREZYMON, Alice, BENANI, Alexandre, PÉNICAUD, Luc, TUDELA FERNÁNDEZ, Raúl, PLANAS OBRADORS, Anna maria, FERNÁNDEZ, Eduardo, CARMONA, Maria del carmen, GOMIS, Ramon. Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers. _PLoS One_. 2012. Vol. 7, núm. 7, pàgs. e39087-1-e39087-12. [consulta: 26 de febrer de 2026]. ISSN: 1932-6203. [Disponible a: https://hdl.handle.net/2445/114289]

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