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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/178246
Dielectric properties and lamellarity of single liposomes measured by in-liquid scanning dielectric microscopy
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Liposomes are widely used as drug delivery carriers and as cell model systems. Here, we measure the dielectric properties of individual liposomes adsorbed on a metal electrode by in-liquid scanning dielectric microscopy in force detection mode. From the measurements the lamellarity of the liposomes, the separation between the lamellae and the specifc capacitance of the lipid bilayer can be obtained. As application we considered the case of non-extruded DOPC liposomes with radii in the range ~100-800 nm. Uni-, bi- and tri-lamellar liposomes have been identifed, with the largest population corresponding to bi-lamellar liposomes. The interlamellar separation in the bi-lamellar liposomes is found to be below ~10 nm in most instances. The specifc capacitance of the DOPC lipid bilayer is found to be ~0.75 µF/cm2 in excellent agreement with the value determined on solid supported planar lipid bilayers. The lamellarity of the DOPC liposomes shows the usual correlation with the liposome's size. No correlation is found, instead, with the shape of the adsorbed liposomes. The proposed approach ofers a powerful label-free and non invasive method to determine the lamellarity and dielectric properties of single liposome
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DI MUZIO, Martina, MILLÁN SOLSONA, Rubén, DOLS-PÉREZ, Aurora, BORRELL HERNÁNDEZ, Jordi, FUMAGALLI, Laura, GOMILA LLUCH, Gabriel. Dielectric properties and lamellarity of single liposomes measured by in-liquid scanning dielectric microscopy. _Journal of Nanobiotechnology_. 2021. Vol. 19, núm. 167. [consulta: 23 de gener de 2026]. ISSN: 1477-3155. [Disponible a: https://hdl.handle.net/2445/178246]