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cc-by-nc-nd (c) Elsevier B.V., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/194057

Concentration methods for the quantification of coronavirus and other potentially pandemic enveloped virus from wastewater

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As the novel SARS-CoV-2 was detected in faeces, environmental researchers have been using centrifugal ultrafiltration, polyethylene glycol precipitation and aluminium hydroxide flocculation to describe its presence in wastewater samples. High recoveries (up to 65%) are described with electronegative filtration when using surrogate viruses, but few literature reports recovery efficiencies using accurate quantification of enveloped viruses. Considering that every single virus will have a different behaviour during viral concentration, it is recommended to use an enveloped virus, and if possible, a betacoronaviruses as murine hepatitis virus, as a surrogate. In this review, we show new data from a newly available technology that provides a quick ultrafiltration protocol for SARSCoV- 2. Wastewater surveillance is an efficient system for the evaluation of the relative prevalence of SARS-CoV-2 infections in a community, and there is the need of using reliable concentration methods for an accurate and sensitive quantification of the virus in water.

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RUSIÑOL ARANTEGUI, Marta, MARTÍNEZ-PUCHOL, Sandra, FORÉS, Eva, ITARTE, Marta, GIRONÈS LLOP, Rosina, BOFILL MAS, Silvia. Concentration methods for the quantification of coronavirus and other potentially pandemic enveloped virus from wastewater. _Current Opinion in Environmental Science & Health_. 2020. Vol. 17, núm. 21-28. [consulta: 25 de gener de 2026]. ISSN: 2468-5844. [Disponible a: https://hdl.handle.net/2445/194057]

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