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2019-01-01

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/132306

Detection of the novel optrA gene among linezolid-resistant enterococci in Barcelona, Spain

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https://doi.org/10.1089/mdr.2018.0028

Resum

The purpose of this study was to describe the presence of the novel optrA gene among clinical isolates of enterococci in a Spanish teaching hospital (May 2016-April 2017). optrA and cfr genes were screened by PCR in all isolates showing linezolid minimal inhibitory concentration (MIC) ≥4 mg/L. The genetic relatedness of the isolates, the presence of resistance and virulence genes, and the genetic environment of optrA were assessed by whole-genome sequencing (WGS). Six of 1,640 enterococci had linezolid MIC ≥4 mg/L. Among them, the optrA gene was detected in five Enterococcus faecalis isolated from unrelated patients. Although none of them had received linezolid or chloramphenicol, all had antecedents of recent quinolone consumption. WGS analysis revealed the existence of two different genotypes: ST585 and ST474. cfr was not detected in any of the isolates. No mutations were detected among the 23S ribosomal RNA and the ribosomal proteins L3, L4, and L22. Both genotypes also carried genes related to aminoglycoside, lincosamide, macrolide, phenicol, and tetracycline resistance. Detection of optrA in a setting with low linezolid consumption and among patients without antecedents of oxazolidinone therapy is of concern.

Matèries

Malalties bacterianes grampositives, Medicaments antibacterians, Genètica bacteriana, Resistència als medicaments, Barcelona (Catalunya)

Matèries (anglès)

Gram-positive bacterial infections, Antibacterial agents, Bacterial genetics, Drug resistance, Barcelona (Catalonia)

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Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citació

CÀMARA, Jordi, CAMOEZ, Mariana, TUBAU, Fe, PUJOL ROJO, Miquel, AYATS, Josefina, ARDANUY TISAIRE, María carmen, DOMÍNGUEZ LUZÓN, Ma. ángeles (maría ángeles). Detection of the novel optrA gene among linezolid-resistant enterococci in Barcelona, Spain. _Microbial Drug Resistance_. 2019. Vol. 25, núm. 1, pàgs. 87-93. [consulta: 25 de febrer de 2026]. ISSN: 1076-6294. [Disponible a: https://hdl.handle.net/2445/132306]

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