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2025

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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/218141

Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization.

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https://doi.org/10.18176/jiaci.0995

Abstract

Patients sensitized to Lipid transfer protein (LTP) were classified as anaphylaxis or sensitized depending on the symptoms elicited by LTP-containing food. CD34+-derived MCs from patients and controls were obtained, sensitized with pooled sera, and challenged with Pru p 3 (peach LTP). Degranulation, PGD2, and cytokine/chemokine release were measured. The TFH13 population was examined by flow cytometry in the peripheral blood of all groups. In parallel, LAD2 cells were activated similarly to patients’ MCs. A distinguishable pattern of mast cell activation was found in anaphylaxis compared to sensitized patients. Robust degranulation, PGD2, and IL-8 and GM-CSF secretion were higher in anaphylaxis, whereas TFG-beta and CCL2 secretion increased in sensitized patients. Concomitantly, anaphylaxis patients had a larger TFH13 population. MC activation profile was dependent on the sera rather than the MC source. In agreement with that, LAD2 cells reproduce the same pattern as MCs from anaphylactic and sensitized patients. The distinct profile of mast cell activation allows for the discrimination between anaphylaxis and sensitized patients. Pooled sera may determine mast cell activation independently of mast cell origin. Besides, the presence of TFH13 cells in anaphylaxis patients points to an essential role of IgE affinity.

Subject

Al·lèrgia alimentària, Inflamació, Mastòcits, Anafilaxi

Subject (English)

Food allergy, Inflammation, Mast cells, Anaphylaxis

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Articles publicats en revistes (Medicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

Citation

OLLÉ, Laia, et al. Mast Cell Activation Profile and TFH13 Detection Discriminate Food Anaphylaxis Versus Sensitization. Journal of Investigational Allergology and Clinical Immunology. 2024. Vol. 35, num. 3. ISSN 1018-9068. [consulted: 10 of August of 2026]. Available at: https://hdl.handle.net/2445/218141

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