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2015-06-04

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cc by (c) Mongui et al., 2015
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/69246

Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries

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http://dx.doi.org/10.1186/s12936-015-0748-6

Abstract

BACKGROUND: The chemical treatment of Plasmodium falciparum for human infections is losing efficacy each year due to the rise of resistance. One possible strategy to find novel anti-malarial drugs is to access the largest reservoir of genomic biodiversity source on earth present in metagenomes of environmental microbial communities. METHODS: A bioluminescent P. falciparum parasite was used to quickly detect shifts in viability of microcultures grown in 96-well plates. A synthetic gene encoding the Dermaseptin 4 peptide was designed and cloned under tight transcriptional control in a large metagenomic insert context (30 kb) to serve as proof-of-principle for the screening platform. RESULTS: Decrease in parasite viability consistently correlated with bioluminescence emitted from parasite microcultures, after their exposure to bacterial extracts containing a plasmid or fosmid engineered to encode the Dermaseptin 4 anti-malarial peptide. CONCLUSIONS: Here, a new technical platform to access the anti-malarial potential in microbial environmental metagenomes has been developed.

Subject

Genòmica, Genètica microbiana, Malària, Plasmodium falciparum, Resistència als medicaments, Efecte dels medicaments sobre els microorganismes

Subject (English)

Genomics, Microbial genetics, Malaria, Plasmodium falciparum, Drug resistance, Effect of drugs on microorganisms

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Articles publicats en revistes (ISGlobal)
Publicacions de projectes de recerca finançats per la UE

Citation

MONGUI, Alvaro, et al. Development of a genetic tool for functional screening of
                anti-malarial bioactive extracts in metagenomic libraries. Malaria Journal. 2015. Vol. 14, num. 233, pags. 1-6. ISSN 1475-2875. [consulted: 19 of August of 2026]. Available at: https://hdl.handle.net/2445/69246

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