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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/69246
Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries
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BACKGROUND: The chemical treatment of Plasmodium falciparum for
human infections is losing efficacy each year due to the rise of
resistance. One possible strategy to find novel anti-malarial
drugs is to access the largest reservoir of genomic biodiversity
source on earth present in metagenomes of environmental
microbial communities. METHODS: A bioluminescent P. falciparum
parasite was used to quickly detect shifts in viability of
microcultures grown in 96-well plates. A synthetic gene encoding
the Dermaseptin 4 peptide was designed and cloned under tight
transcriptional control in a large metagenomic insert context
(30 kb) to serve as proof-of-principle for the screening
platform. RESULTS: Decrease in parasite viability consistently
correlated with bioluminescence emitted from parasite
microcultures, after their exposure to bacterial extracts
containing a plasmid or fosmid engineered to encode the
Dermaseptin 4 anti-malarial peptide. CONCLUSIONS: Here, a new
technical platform to access the anti-malarial potential in
microbial environmental metagenomes has been developed.
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MONGUI, Alvaro, PÉREZ-LLANOS, Francy j., YAMAMOTO, Marcio m., LOZANO, Marcela, ZAMBRANO, Maria m., PORTILLO, Patricis del, FERNÁNDEZ BECERRA, María del carmen, RESTREPO, Silvia, JUNCA, Howard. Development of a genetic tool for functional screening of
anti-malarial bioactive extracts in metagenomic libraries. _Malaria Journal_. 2015. Vol. 14, núm. 233, pàgs. 1-6. [consulta: 25 de febrer de 2026]. ISSN: 1475-2875. [Disponible a: https://hdl.handle.net/2445/69246]