Salmosan, a β-Galactomannan-Rich Product, Protects Epithelial Barrier Function in Caco-2 Cells Infected by Salmonella enterica Serovar Enteritidis

dc.contributor.authorBrufau Bonet, M. Teresa (Maria Teresa)
dc.contributor.authorCampo Sabariz, Joan
dc.contributor.authorBou Novensà, Ricard
dc.contributor.authorCarné, S.
dc.contributor.authorBrufau, Joaquim
dc.contributor.authorVilà, Borja
dc.contributor.authorMarqués Villavecchia, Ana M.
dc.contributor.authorGuardiola Ibarz, Francesc
dc.contributor.authorFerrer i Roig, Ruth
dc.contributor.authorMartín Venegas, Raquel
dc.date.accessioned2020-06-09T07:26:28Z
dc.date.available2020-06-09T07:26:28Z
dc.date.issued2016-06-29
dc.date.updated2020-06-09T07:26:29Z
dc.description.abstractBackground: One promising strategy for reducing human salmonellosis induced by Salmonella Enteritidis is to supplement animal diets with natural feed additives such as mannan oligosaccharides (MOS). Objective: The aim of this study was to investigate the potential role of Salmosan® (S-βGM), an extremely β-galactomannan-rich MOS product, in preventing epithelial barrier function disruption induced by Salmonella Enteritidis colonization in an in vitro model of intestinal Caco-2 cells in culture. Methods: Differentiated Caco-2 cells were incubated for 3 h with Salmonella Enteritidis at a multiplicity of infection 10 (MOI 10) in the absence or presence of 500 µg/mL S-βGM. Paracellular permeability (PP) was assessed by transepithelial electrical resistance (TER), D-mannitol and fluorescein isothiocyanate-dextran (FD-4) flux. Tight junction (TJ) proteins and cytoskeletal actin were also localized by confocal microscopy. Reactive oxygen species (ROS) and lipid peroxidation products were evaluated. Scanning and transmission electron microscopy were used to visualize Salmonella Enteritidis adhesion to, and invasion of, the Caco-2 cell cultures. Results: Compared to controls, TER was significantly reduced 30 %, and D-mannitol and FD-4 flux were significantly increased 374 % and 54 % in Salmonella Enteritidis-infected cultures. The presence of S-βGM in infected cultures induced total recoveries of TER and FD-4 flux to values that did not differ from control (P = 0.07 and P = 0.55, respectively), and a partial recovery of D-mannitol flux. These effects were confirmed by immunolocalization of actin, zonula occludens protein-1 (ZO-1) and occludin. Similar results were obtained for Salmonella Dublin. The protection of S-βGM on PP in infected cultures may be associated with a total recovery of ROS production to values that did not differ from control (P = 0.11). Moreover, S-βGM has the capacity to agglutinate bacteria, leading to a significant reduction in intracellular Salmonella Enteritidis of 32 % (P < 0.05). Conclusions: The results demonstrate that S-βGM contributes to protecting epithelial barrier function in a Caco-2 cell model disrupted by Salmonella Enteritidis.
dc.format.extent7 p.
dc.format.mimetypeapplication/pdf
dc.identifier.idgrec663165
dc.identifier.issn0022-3166
dc.identifier.urihttps://hdl.handle.net/2445/164879
dc.language.isoeng
dc.publisherAmerican Society for Nutrition
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.3945/jn.116.232546
dc.relation.ispartofJournal of Nutrition, 2016, vol. 146, num. 8, p. 1492-1498
dc.relation.urihttps://doi.org/10.3945/jn.116.232546
dc.rights(c) American Society for Nutrition, 2016
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.sourceArticles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)
dc.subject.classificationSalmonel·la
dc.subject.classificationFarmacologia
dc.subject.classificationFisiologia patològica
dc.subject.classificationFarmacocinètica
dc.subject.classificationSuplements nutritius
dc.subject.classificationMicrobiologia
dc.subject.classificationOligosacàrids
dc.subject.classificationCèl·lules epitelials
dc.subject.classificationPermeabilitat
dc.subject.otherSalmonella
dc.subject.otherPharmacology
dc.subject.otherPathological physiology
dc.subject.otherPharmacokinetics
dc.subject.otherDietary supplements
dc.subject.otherMicrobiology
dc.subject.otherOligosaccharides
dc.subject.otherEpithelial cells
dc.subject.otherPermeability
dc.titleSalmosan, a β-Galactomannan-Rich Product, Protects Epithelial Barrier Function in Caco-2 Cells Infected by Salmonella enterica Serovar Enteritidis
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/acceptedVersion

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