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Martia_ez-VendrellX_MalarJ_2020.pdf (1.1 MB)

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2020-01-09

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cc by (c) Martiáñez Vendrell et al., 2020
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/150434

Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma

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http://dx.doi.org/10.1186/s12936-019-3083-5

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Background: Malaria diagnostics by rapid diagnostic test (RDT) relies primarily on the qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and Plasmodium spp lactate dehydrogenase (pLDH). As novel RDTs with increased sensitivity are being developed and implemented as point of care diagnostics, highly sensitive laboratory-based assays are needed for evaluating RDT performance. Here, a quantitative suspension array technol‑ ogy (qSAT) was developed, validated and applied for the simultaneous detection of PfHRP2 and pLDH in a variety of biological samples (whole blood, plasma and dried blood spots) from individuals living in diferent endemic countries. Results: The qSAT was specifc for the target antigens, with analytical ranges of 6.8 to 762.8 pg/ml for PfHRP2 and 78.1 to 17076.6 pg/ml for P. falciparum LDH (Pf-LDH). The assay detected Plasmodium vivax LDH (Pv-LDH) at a lower sensitivity than Pf-LDH (analytical range of 1093.20 to 187288.5 pg/ml). Both PfHRP2 and pLDH levels determined using the qSAT showed to positively correlate with parasite densities determined by quantitative PCR (Spearman r=0.59 and 0.75, respectively) as well as microscopy (Spearman r=0.40 and 0.75, respectively), suggesting the assay to be a good predictor of parasite density. Conclusion: This immunoassay can be used as a reference test for the detection and quantifcation of PfHRP2 and pLDH, and could serve for external validation of RDT performance, to determine antigen persistence after parasite clearance, as well as a complementary tool to assess malaria burden in endemic settings.

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Malària, Plasma sanguini

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Malaria, Blood plasma

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Articles publicats en revistes (ISGlobal)

Citació

MARTIÁÑEZ VENDRELL, Xavier, JIMÉNEZ, Alfons, VÁSQUEZ, Ana, CAMPILLO, Ana, INCARDONA, Sandra, GONZÁLEZ, Raquel, GAMBOA, Dionicia, TORRES, Katherine, OYIBO, Wellington, FAYE, Babacar, MACETE, Eusebio víctor, MENÉNDEZ, Clara, DING, Xavier c., MAYOR APARICIO, Alfredo gabriel. Quantification of malaria antigens PfHRP2 and pLDH by
                quantitative suspension array technology in whole blood, dried
                blood spot and plasma. _Malaria Journal_. 2020. Vol. 19. [consulta: 25 de febrer de 2026]. ISSN: 1475-2875. [Disponible a: https://hdl.handle.net/2445/150434]

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