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gomes2016_2244.pdf (1.35 MB)

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2016-09-14

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cc by (c) Gomes et al., 2016
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/102466

Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis

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Recurs relacionat

http://dx.doi.org/10.1371/journal.pntd.0004989

Resum

BACKGROUND: Bartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas. METHODOLOGY/PRINCIPAL FINDINGS: Blood (n = 198) and serum (n = 177) samples were collected in northern Peru. Clinical data were recorded. Specific 16S rRNA amplification by RT-PCR, IFA and ELISA for IgM/IgG with whole cells as antigens was done. Western blot analysis and N-terminal amino acid sequencing detected seroreactive proteins. ELISAs for IgM/IgG for the antigenic candidates were performed. Of the population 33.3% reported at least one symptom compatible with Carrion's disease; 25.4% (IFA), 27.1% (ELISA-IgG), 33.9% (ELISA-IgM) and 38.9% (RT-PCR) of samples were positive. Four proteins were considered potential antigenic candidates, including two new antigenic candidates, succinyl-CoA synthetase subunit alpha (SCS-alpha) and succinyl-CoA synthetase subunit beta (SCS-beta). On Western blot both Pap31 and SCS-alpha interacted with IgM, while GroEL and SCS-beta interacted with IgG. The presence of specific antibodies against the antigenic candidates varied from 34.5% (IgG against SCS-alpha) to 97.2% (IgM against Pap31). CONCLUSIONS/SIGNIFICANCE: RT-PCR and the high levels of positivity for specific ELISAs demonstrate high levels of B. bacilliformis exposure and asymptomatic carriers among inhabitants. The new antigens identified might be used as a new rapid diagnostic tool to diagnose acute Carrion's disease and identify asymptomatic carriers.

Matèries

Malalties bacterianes, Paisatge rural, Antígens bacterians

Matèries (anglès)

Bacterial diseases, Rural landscape, Bacterial antigens

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Articles publicats en revistes (ISGlobal)

Citació

GOMES, Cláudia, et al. Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella
                bacilliformis. PLoS Neglected Tropical Diseases. 2016. Vol. 10, num. 9, pags. e0004989. ISSN 1935-2727. [consulted: 14 of August of 2026]. Available at: https://hdl.handle.net/2445/102466

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