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cc-by (c) Costa, Rita et al., 2018
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/134823

Vertebrate SLRP family evolution and the subfunctionalization of osteoglycin gene duplicates in teleost fish

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Background Osteoglycin (OGN, a.k.a. mimecan) belongs to cluster III of the small leucine-rich proteoglycans (SLRP) of the extracellular matrix (ECM). In vertebrates OGN is a characteristic ECM protein of bone. In the present study we explore the evolution of SLRP III and OGN in teleosts that have a skeleton adapted to an aquatic environment. Results The SLRP gene family has been conserved since the separation of chondrichthyes and osteichthyes. Few gene duplicates of the SLRP III family exist even in the teleosts that experienced a specific whole genome duplication. One exception is ogn for which duplicate copies were identified in fish genomes. The ogn promoter sequence and in vitro mesenchymal stem cell (MSC) cultures suggest the duplicate ogn genes acquired divergent functions. In gilthead sea bream (Sparus aurata) ogn1 was up-regulated during osteoblast and myocyte differentiation in vitro, while ogn2 was severely down-regulated during bone-derived MSCs differentiation into adipocytes in vitro. Conclusions Overall, the phylogenetic analysis indicates that the SLRP III family in vertebrates has been under conservative evolutionary pressure. The retention of the ogn gene duplicates in teleosts was linked with the acquisition of different functions. The acquisition by OGN of functions other than that of a bone ECM protein occurred early in the vertebrate lineage

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COSTA, Rita a., MARTINS, Rute s. t., CAPILLA CAMPOS, Encarnación, ANJOS, L., POWER, Deborah m.. Vertebrate SLRP family evolution and the subfunctionalization of osteoglycin gene duplicates in teleost fish. _BMC Developmental Biology_. 2018. Vol. 18, núm. 191. [consulta: 21 de gener de 2026]. ISSN: 1471-213X. [Disponible a: https://hdl.handle.net/2445/134823]

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