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2014

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cc-by (c) Egaña Gorroño, Lander et al., 2014
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/120810

Differential microRNA expression profile between stimulated PBMCs from HIV-1 infected elite controllers and viremic progressors

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https://doi.org/10.1371/journal.pone.0106360

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BACKGROUND: The emerging relationship between microRNAs (miRNA) and viral-control is a topic of interest in the field of HIV. Host-genome might play an important role in the control of viremia. The aim of this study was to assess the specific miRNA profile that could contribute to the control of HIV replication in Elite Controllers. RESULTS: After adequate normalization, expression profile of 286 human miRNAs (hsa-miR) was evaluated in phytohaemagglutinin-stimulated PBMCs from 29 individuals classified in 4 groups: 8 elite controllers (EC; viral load <50 cp/ml without treatment), 8 viremic progressors (VP; VL>5000 cp/ml without treatment), 8 patients under antiretroviral treatment (ART; VL<200 cp/ml) and 5 uninfected individuals (HIV-) through TaqMan Array Human microRNA Cards v3.0. A differential expression pattern consisting of 23 miRNAs became significantly different when comparing EC and VP. Profiling analysis segregated the population in two different blocks: while EC and HIV- clustered together in the same block (EC/HIV-_block 1), VP and ART individuals clustered together in a second block (VP/ART_block 2). Two inversely expressed miRNA patterns were determined within those two blocks: a set of 4 miRNAs (hsa-miR-221, -27a, -27b and -29b) was up-expressed in EC/HIV-_block and down-expressed in VP/ART_block while 19 miRNAs were down-expressed in block 1 and up-expressed in block 2. Differential miRNAs were successfully validated through individual RT-qPCR assays. CONCLUSIONS: Profile in EC resembled HIV- and differentially clusters with VP and ART. Therefore, differential clustering does not rely on undetectable viremia.

Matèries

Expressió gènica, Micro RNAs, Infeccions per VIH, VIH (Virus)

Matèries (anglès)

Gene expression, MicroRNAs, HIV infections, HIV (Viruses)

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Articles publicats en revistes (Medicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

Citació

EGAÑA GORROÑO, Lander, ESCRIBÀ, Tuixent, BOULANGER, Nicolas, CRESPO GUARDO, Alberto, LEÓN GARCÍA, Agathe, BARGALLÓ, Manel enric, GARCÍA, Felipe, GATELL, José m., PLANA PRADES, Montserrat, ARNEDO, Mireia. Differential microRNA expression profile between stimulated PBMCs from HIV-1 infected elite controllers and viremic progressors. _PLoS One_. 2014. Vol. 9, núm. 9, pàgs. e106360. [consulta: 20 de gener de 2026]. ISSN: 1932-6203. [Disponible a: https://hdl.handle.net/2445/120810]

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