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589370.pdf (1.47 MB)

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2010

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cc-by-nc (c) Zaouali, Mohamed Amine et al., 2010
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/189205

Hypoxia inducible factor-1α accumulation in steatotic liver preservation: role of nitric oxide

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Recurs relacionat

https://doi.org/10.3748/wjg.v16.i28.3499

Resum

AIM: To examine the relevance of hypoxia inducible factor (HIF-1) and nitric oxide (NO) on the preservation of fatty liver against cold ischemia-reperfusion injury (IRI). METHODS: We used an isolated perfused rat liver model and we evaluated HIF-1α in steatotic and non-steatotic livers preserved for 24 h at 4°C in University of Wisconsin and IGL-1 solutions, and then subjected to 2 h of normothermic reperfusion. After normoxic reperfusion, liver enzymes, bile production, bromosulfophthalein clearance, as well as HIF-1α and NO [endothelial NO synthase (eNOS) activity and nitrites/nitrates] were also measured. Other factors associated with the higher susceptibility of steatotic livers to IRI, such as mitochondrial damage and vascular resistance were evaluated. RESULTS: A significant increase in HIF-1α was found in steatotic and non-steatotic livers preserved in IGL-1 after cold storage. Livers preserved in IGL-1 showed a significant attenuation of liver injury and improvement in liver function parameters. These benefits were enhanced by the addition of trimetazidine (an anti-ischemic drug), which induces NO and eNOS activation, to IGL-1 solution. In normoxic reperfusion, the presence of NO favors HIF-1α accumulation, promoting also the activation of other cytoprotective genes, such as heme-oxygenase-1. CONCLUSION: We found evidence for the role of the HIF-1α/NO system in fatty liver preservation, especially when IGL-1 solution is used.

Matèries

Malalties del fetge, Òxid nítric, Oxigen en l'organisme, Conservació d'òrgans

Matèries (anglès)

Liver diseases, Nitric oxide, Oxygen in the body, Preservation of organs

Citació

Col·leccions

Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

Citació

ZAOUALI, Mohamed Amine, et al. Hypoxia inducible factor-1α accumulation in steatotic liver preservation: role of nitric oxide. World Journal of Gastroenterology. 2010. Vol. 16, num. 28, pags. 3499-3509. ISSN 1007-9327. [consulted: 14 of August of 2026]. Available at: https://hdl.handle.net/2445/189205

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