Miniatura

Fitxers

206625.pdf (963.45 KB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2017-06-04

Llicència de publicació

cc-by (c) Morén, C. et al., 2017
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/219047

Colonic oxidative and mitochondrial function in Parkinson's disease and idiopathic REM sleep behavior disorder

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1155/2017/9816095

Resum

Objective: To determine potential mitochondrial and oxidative alterations in colon biopsies from idiopathic REM sleep behavior disorder (iRBD) and Parkinson's disease (PD) subjects. Methods: Colonic biopsies from 7 iRBD subjects, 9 subjects with clinically diagnosed PD, and 9 healthy controls were homogenized in 5% w/v mannitol. Citrate synthase (CS) and complex I (CI) were analyzed spectrophotometrically. Oxidative damage was assessed either by lipid peroxidation, through malondialdehyde and hydroxyalkenal content by spectrophotometry, or through antioxidant enzyme levels of superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (Gpx1), and catalase (CAT) by western blot. The presence of mitochondrial DNA (mtDNA) deletions was assessed by long PCR and electrophoresis. Results: Nonsignificant trends to CI decrease in both iRBD (45.69 ± 18.15; 23% decrease) and PD patients (37.57 ± 12.41; 37% decrease) were found compared to controls (59.51 ± 12.52, p: NS). Lipid peroxidation was maintained among groups (iRBD: 27.46 ± 3.04, PD: 37.2 ± 3.92, and controls: 31.71 ± 3.94; p: NS). Antioxidant enzymes SOD2 (iRBD: 2.30 ± 0.92, PD: 1.48 ± 0.39, and controls: 1.09 ± 0.318) and Gpx1 (iRBD 0.29 ± 0.12, PD: 0.56 ± 0.33, and controls: 0.38 ± 0.16) did not show significant differences between groups. CAT was only detected in 2 controls and 1 iRBD subject. One iRBD patient presented a single mtDNA deletion.

Matèries

Malaltia de Parkinson, Biòpsia, Còlon, Trastorns del son

Matèries (anglès)

Parkinson's disease, Biopsy, Colon, Sleep disorders

Citació

Col·leccions

Articles publicats en revistes (Medicina)

Citació

NAVARRO OTANO, Judith, MORÉN NÚÑEZ, Constanza, GONZÁLEZ CASACUBERTA, Ingrid, JUÁREZ FLORES, Diana luz, VILAS ROLÁN, Dolores, GARRABOU TORNOS, Glòria, MILISENDA, José, PONT SUNYER, Claustre, CATALÁN GARCÍA, Marc, GUITART MAMPEL, Mariona, TOBÍAS, Ester, CARDELLACH, Francesc, VALLDEORIOLA SERRA, Francesc, IRANZO, Alex, TOLOSA, Eduardo. Colonic oxidative and mitochondrial function in Parkinson's disease and idiopathic REM sleep behavior disorder. _Parkinson's Disease_. 2017. [consulta: 21 de gener de 2026]. ISSN: 2042-0080. [Disponible a: https://hdl.handle.net/2445/219047]

Exportar metadades

JSON - METS

Compartir registre