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2018-02-13

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cc-by (c) López Begines, Santiago et al., 2018
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/131133

Molecular determinants of guanylate cyclase activating protein subcellular distribution in photoreceptor cells of the retina

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https://doi.org/10.1038/s41598-018-20893-1

Abstract

Retinal guanylate cyclase (RetGC) and guanylate cyclase activating proteins (GCAPs) play an important role during the light response in photoreceptor cells. Mutations in these proteins are linked to distinct forms of blindness. RetGC and GCAPs exert their role at the ciliary outer segment where phototransduction takes place. We investigated the mechanisms governing GCAP1 and GCAP2 distribution to rod outer segments by expressing selected GCAP1 and GCAP2 mutants as transient transgenes in the rods of GCAP1/2 double knockout mice. We show that precluding GCAP1 direct binding to RetGC (K23D/GCAP1) prevented its distribution to rod outer segments, while preventing GCAP1 activation of RetGC post-binding (W94A/GCAP1) did not. We infer that GCAP1 translocation to the outer segment strongly depends on GCAP1 binding affinity for RetGC, which points to GCAP1 requirement to bind to RetGC to be transported. We gain further insight into the distinctive regulatory steps of GCAP2 distribution, by showing that a phosphomimic at position 201 is sufficient to retain GCAP2 at proximal compartments; and that the bovine equivalent to blindness-causative mutation G157R/GCAP2 results in enhanced phosphorylation in vitro and significant retention at the inner segment in vivo, as likely contributing factors to the pathophysiology.

Subject

Fotoreceptors, Retina, Malalties de la retina, Ceguesa, Transformació genètica

Subject (English)

Photoreceptors, Retina, Retinal diseases, Blindness, Genetic transformation

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Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citation

LÓPEZ BEGINES, Santiago, PLANA-BONAMAISÓ, Anna and MÉNDEZ ZUNZUNEGUI, Ana. Molecular determinants of guanylate cyclase activating protein subcellular distribution in photoreceptor cells of the retina. Scientific Reports. 2018. Vol. 8, num. 2903. ISSN 2045-2322. [consulted: 15 of August of 2026]. Available at: https://hdl.handle.net/2445/131133

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