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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/113245
Transgenic expression of soluble human CD5 enhances experimentally-induced autoimmune and anti-tumoral immune responses
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CD5 is a lymphoid-specific transmembrane glycoprotein constitutively expressed on thymocytes and mature T and B1a lymphocytes. Current data support the view that CD5 is a negative regulator of antigen-specific receptor-mediated signaling in these cells, and that this would likely be achieved through interaction with CD5 ligand/s (CD5L) of still undefined nature expressed on immune or accessory cells. To determine the functional consequence of loss of CD5/CD5L interaction in vivo, a new transgenic mouse line was generated (shCD5EμTg), expressing a circulating soluble form of human CD5 (shCD5) as a decoy to impair membrane-bound CD5 function. These shCD5EμTg mice showed an enhanced response to autologous antigens, as deduced from the presentation of more severe forms of experimentally inducible autoimmune disease (collagen-induced arthritis, CIA; and experimental autoimmune encephalitis, EAE), as well as an increased anti-tumoral response in non-orthotopic cancer models (B16 melanoma). This enhancement of the immune response was in agreement with the finding of significantly reduced proportions of spleen and lymph node Treg cells (CD4+CD25+FoxP3+), and of peritoneal IL-10-producing and CD5+ B cells, as well as an increased proportion of spleen NKT cells in shCD5EμTg mice. Similar changes in lymphocyte subpopulations were observed in wild-type mice following repeated administration of exogenous recombinant shCD5 protein. These data reveal the relevant role played by CD5/CD5L interactions on the homeostasis of some functionally relevant lymphocyte subpopulations and the modulation of immune responses to autologous antigens.
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FENUTRÍA, Rafael, MARTINEZ, Vanesa gabriela, SIMÕES, Inês, POSTIGO, Jorge, GIL, Victor, MARTÍNEZ-FLORENSA, Mario, SINTES, Jordi, NAVES, Rodrigo, CASHMAN, Kevin s., ALBEROLA-ILA, José, RAMOS CASALS, Manuel, SOLDEVILA, Gloria, RAMAN, Chander, MERINO, Jesús, MERINO, Ramón, ENGEL ROCAMORA, Pablo, LOZANO SOTO, Francisco. Transgenic expression of soluble human CD5 enhances experimentally-induced autoimmune and anti-tumoral immune responses. _PLoS One_. 2014. Vol. 9, núm. 1, pàgs. e84895. [consulta: 15 de gener de 2026]. ISSN: 1932-6203. [Disponible a: https://hdl.handle.net/2445/113245]