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2014-01-09

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cc-by (c) Bisoffi, Zeno et al., 2014
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/175538

Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection.

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https://doi.org/10.1371/journal.pntd.0002640

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Background: The diagnosis of Strongyloides stercoralis (S. stercoralis) infection is hampered by the suboptimal sensitivity of fecal-based tests. Serological methods are believed to be more sensitive, although assessing their accuracy is difficult because of the lack of sensitivity of a fecal-based reference ('gold') standard. Methods: The sensitivity and specificity of 5 serologic tests for S. stercoralis (in-house IFAT, NIE-ELISA and NIE-LIPS and the commercially available Bordier-ELISA and IVD-ELISA) were assessed on 399 cryopreserved serum samples. Accuracy was measured using fecal results as the primary reference standard, but also using a composite reference standard (based on a combination of tests). Results: According to the latter standard, the most sensitive test was IFAT, with 94.6% sensitivity (91.2-96.9), followed by IVD-ELISA (92.3%, 87.7-96.9). The most specific test was NIE-LIPS, with specificity 99.6% (98.9-100), followed by IVD-ELISA (97.4%, 95.5-99.3). NIE-LIPS did not cross-react with any of the specimens from subjects with other parasitic infections. NIE-LIPS and the two commercial ELISAs approach 100% specificity at a cut off level that maintains ≥70% sensitivity. Conclusions: NIE-LIPS is the most accurate serologic test for the diagnosis of S. stercoralis infection. IFAT and each of the ELISA tests are sufficiently accurate, above a given cut off, for diagnosis, prevalence studies and inclusion in clinical trials.

Matèries

Malalties parasitàries, Larves, Serodiagnòstic

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Parasitic diseases, Larvae, Serodiagnosis

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Articles publicats en revistes (Medicina)

Citació

BISOFFI, Zeno, BUONFRATE, Dora, SEQUI, Marco, MEJIA, Rojelio, CIMINO, Rubén o., KROLEWIECKI, Alejandro j., ALBONICO, Marco, GOBBO, Maria, BONAFINI, Stefania, ANGHEBEN, Andrea, REQUENA-MÉNDEZ, Ana, MUÑOZ GUTIÉRREZ, José, NUTMAN, Thomas b.. Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection.. _PLoS Neglected Tropical Diseases_. 2014. Vol. 8, núm. 1, pàgs. e2640. [consulta: 7 de febrer de 2026]. ISSN: 1935-2735. [Disponible a: https://hdl.handle.net/2445/175538]

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