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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/170448
Chemical Modification of Microcin J25 Reveals New Insights on the Stereospecific Requirements for Antimicrobial Activity
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In this study, microcin J25, a potent antimicrobial lasso peptide that acts on Gram-negative bacteria, was subjected to a harsh treatment with a base in order to interrogate its stability and mechanism of action and explore its structure-activity relationship. Despite the high stability reported for this lasso peptide, the chemical treatment led to the detection of a new product. Structural studies revealed that this product retained the lasso topology, but showed no antimicrobial activity due to the epimerization of a key residue for the activity. Further microbiological assays also demonstrated that it showed a high synergistic effect with colistin.
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MARTÍN GÓMEZ, Helena, et al. Chemical Modification of Microcin J25 Reveals New Insights on the Stereospecific Requirements for Antimicrobial Activity. International Journal Of Molecular Sciences. 2019-10-02. Vol. 20, num. 20, pags. 5152. [consulted: 5 of October of 2026]. Available at: https://hdl.handle.net/2445/170448