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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/174195
Intratumoral heterogeneity and clonal evolution in liver cancer
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Clonal evolution of a tumor ecosystem depends on different selection pressures that are principally immune and treatment mediated. We integrate RNA-seq, DNA sequencing, TCR-seq and SNP array data across multiple regions of liver cancer specimens to map spatio-temporal interactions between cancer and immune cells. We investigate how these interactions reflect intra-tumor heterogeneity (ITH) by correlating regional neo-epitope and viral antigen burden with the regional adaptive immune response. Regional expression of passenger mutations dominantly recruits adaptive responses as opposed to hepatitis B virus and cancer-testis antigens. We detect different clonal expansion of the adaptive immune system in distant regions of the same tumor. An ITH-based gene signature improves single-biopsy patient survival predictions and an expression survey of 38,553 single cells across 7 regions of 2 patients further reveals heterogeneity in liver cancer. These data quantify transcriptomic ITH and how the different components of the HCC ecosystem interact during cancer evolution.
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LOSIC, Bojan, et al. Intratumoral heterogeneity and clonal evolution in liver cancer. Nature Communications. 2020. Vol. 11, num. 291. ISSN 2041-1723. [consulted: 8 of June of 2026]. Available at: https://hdl.handle.net/2445/174195