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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/218913
Development of molecularly imprinted polymers and its magnetic version for the extraction of fluoroquinolones from milk.
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The aim of the present study is to develop selective extracting materials applicable to a diversity of fluoroquinolones. A series of Molecularly Imprinted Materials (MIPs) were prepared in order to choose the nature of the monomer and that of the porogen together with its ratio with the cross-linker. A non-regulated quinolone, Levofloxacin, was used as a template to avoid false positive results in its application. The resulting MIPs were evaluated in MISPE experiments for Enrofloxacin. The polymer prepared using methyl acrylate (MA) as a monomer and MeOH/CHCl3 (1:1, v/v) as a porogen provided the desired selectivity. Concretely, 100 μL (1.1mmol) of MA as a monomer, 1.30 mL (9.8 mmol) of EGDMA (ethyleneglycol imethacrylate) as a cross-linker, 31 mg (0.19 mmol) of AIBN (2,2′-azobis(2-methylpropionitrile)) as an initiator, were mixed with 85 mg (0.24mmol) of levofloxacin as a template and 4 mL of MeOH/CHCl3 (1:1, v/v) as a porogen.The obtained optimized composition was upgraded to magnetic molecularly imprinted polymers (MMIPs) tobe used as a dispersive-SPE extracting materials in the analysis of fluoroquinolones in milk. The selectivity of theresulting material for several fluoroquinolones (enrofloxacin, ciprofloxacin, sarafloxacin and norfloxacin) wasstudied. Finally, the MMIP was tested against real quinolone positive milk samples to evaluate its applicability.
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MEGIAS-PÉREZ, Elena, et al. Development of molecularly imprinted polymers and its magnetic version for the extraction of fluoroquinolones from milk. . Microchemical Journal. 2023. Vol. 195, num. 109422. ISSN 0026-265X. [consulted: 7 of August of 2026]. Available at: https://hdl.handle.net/2445/218913