High-throughput analysis of lipid hydroperoxides in edible oils and fats using the fluorescent reagent diphenyl-1-pyrenylphosphine
| dc.contributor.author | Santas Gutiérrez, Jonathan | |
| dc.contributor.author | Guzmán, Yeimmy J. | |
| dc.contributor.author | Guardiola Ibarz, Francesc | |
| dc.contributor.author | Rafecas Martínez, Magdalena | |
| dc.contributor.author | Bou Novensà, Ricard | |
| dc.date.accessioned | 2020-06-04T09:10:56Z | |
| dc.date.available | 2020-06-04T09:10:56Z | |
| dc.date.issued | 2014-11-01 | |
| dc.date.updated | 2020-06-04T09:10:56Z | |
| dc.description.abstract | A fluorometric method for the determination of hydroperoxides (HP) in edible oils and fats using the reagent diphenyl-1-pyrenylphosphine (DPPP) was developed and validated. Two solvent media containing 100% butanol or a mixture of chloroform/methanol (2:1, v/v) can be used to solubilize lipid samples. Regardless of the solvent used to solubilize the sample, the DPPP method was precise, accurate, sensitive and easy to perform. The HP content of 43 oil and fat samples was determined and the results were compared with those obtained by means of the AOCS Official Method for the determination of peroxide value (PV) and the ferrous oxidation-xylenol orange (FOX) method. The proposed method not only correlates well with the PV and FOX methods, but also presents some advantages such as requiring low sample and solvent amounts and being suitable for high-throughput sample analysis. | |
| dc.format.extent | 7 p. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.idgrec | 642910 | |
| dc.identifier.issn | 0308-8146 | |
| dc.identifier.uri | https://hdl.handle.net/2445/164262 | |
| dc.language.iso | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.foodchem.2014.04.059 | |
| dc.relation.ispartof | Food Chemistry, 2014, vol. 162, p. 235-241 | |
| dc.relation.uri | https://doi.org/10.1016/j.foodchem.2014.04.059 | |
| dc.rights | (c) Elsevier B.V., 2014 | |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.source | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) | |
| dc.subject.classification | Olis i greixos comestibles | |
| dc.subject.classification | Colorants en els aliments | |
| dc.subject.classification | Peròxids | |
| dc.subject.classification | Compostos organofosforats | |
| dc.subject.classification | Reacció d'oxidació-reducció | |
| dc.subject.classification | Fenols | |
| dc.subject.classification | Solubilitat | |
| dc.subject.classification | Dissolvents | |
| dc.subject.other | Edible oils and fats | |
| dc.subject.other | Food colorings | |
| dc.subject.other | Peroxides | |
| dc.subject.other | Organophosphorus compounds | |
| dc.subject.other | Oxidation-reduction reaction | |
| dc.subject.other | Phenols | |
| dc.subject.other | Solubility | |
| dc.subject.other | Solvents | |
| dc.title | High-throughput analysis of lipid hydroperoxides in edible oils and fats using the fluorescent reagent diphenyl-1-pyrenylphosphine | |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/acceptedVersion |
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