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2025-12-01

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cc-by-nc (c) Fernando Duran-Sindreu, et al., 2025
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/227050

A Thermosensitive Gel Containing Biodegradable Nanoparticles Carrying Calcium Hydroxide as Antibacterial Intracanal Therapy

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https://doi.org/10.1111/iej.70032

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Aim: To characterise and evaluate mucoadhesive strength and antibacterial properties of a nanotechnological formulation forendodontic disinfection based on biodegradable nanoparticles dispersed in a thermosensitive gel containing calcium hydroxide(Ca(OH) 2 -NPs-gel).Methodology: Morphology of Ca(OH) 2 -NPs-gel was studied using transmission electron microscopy. Moreover, Ca(OH) 2 -NPs-gel was sterilised using gamma irradiation (25 kGy), and the stability after the sterilisation process was studied by measuringCa(OH) 2 -NPs-gel average size, polydispersity index, zeta potential and encapsulation efficiency. To assess the ex vivo mucoad-hesive strength, extracted single-rooted human teeth were used to measure the force necessary to separate the formulationfrom the teeth. In addition, the short-time stability of Ca(OH) 2 -NPs-gel was evaluated monthly, analysing entrapment efficacy,backscattering and transmittance of Ca(OH) 2 -NPs-gel stored at different temperatures (4°C, 25°C and 37°C). Furthermore, theantibacterial analysis of Ca(OH) 2 -NPs-gel was performed against Enterococcus faecalis inoculated in extracted human single-root teeth and evaluated by confocal and scanning electron microscopy. Finally, the metabolic activity of bacteria was studiedthrough a resazurin assay to evaluate bacterial survival after treatment.Results: Ca(OH) 2 -NPs-gel owned a round shape and a smooth surface without particle aggregation. Sterilisation did not inducean alteration in Ca(OH) 2 -NPs-gel physicochemical properties and Ca(OH) 2 -NPs-gel presented a high adhesion strength. In addi-tion, 4°C was the best temperature to store Ca(OH) 2 -NPs-gel. Regarding the antibacterial therapeutic efficacy, Ca(OH) 2 -NPs-gelpossesses suitable antibacterial properties, indicating that it efficiently reduces bacterial biofilms.Conclusion: Calcium hydroxide-loaded PLGA nanoparticles dispersed in a thermosensitive gel have been developed, optimisedand characterised, obtaining excellent antibacterial properties and achieving bacterial disinfection levels similar to those ofcommercial formulations.

Matèries

Nanopartícules, Medicaments antibacterians, Endodòncia

Matèries (anglès)

Nanoparticles, Antibacterial agents, Endodontics

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Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)

Citació

DURAN-SINDREU, Fernando, et al. A Thermosensitive Gel Containing Biodegradable Nanoparticles Carrying Calcium Hydroxide as Antibacterial Intracanal Therapy. International Endodontic Journal. 2025. ISSN 0143-2885. [consulted: 3 of August of 2026]. Available at: https://hdl.handle.net/2445/227050

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