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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/125974
Artilysation' of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci
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Endolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain a new generation of lytic proteins, Artilysins, with specific activity against Gram-negative bacteria. Here, we demonstrate that artilysation can also be used to enhance the antibacterial activity of endolysins against Gram-positive bacteria and to reduce the dependence on external conditions. Art-240, a chimeric protein of the anti-streptococcal endolysin λSa2lys and the polycationic peptide PCNP, shows a similar species specificity as the parental endolysin, but the bactericidal activity against streptococci increases and is less affected by elevated NaCl concentrations and pH variations. Time-kill experiments and time-lapse microscopy demonstrate that the killing rate of Art-240 is approximately two-fold higher compared to wildtype endolysin λSa2lys, with a reduction in viable bacteria of 3 log units after 10min. In addition, lower doses of Art240 are required to achieve the same bactericidal effect.
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RODRÍGUEZ-RUBIO, Lorena, CHANG, Wai-ling, GUTIÉRREZ, Diana, LAVIGNE, Rob, MARTÍNEZ, Beatriz, RODRÍGUEZ, Ana, GOVERS, Sander k., AERTSEN, Abram, HIRL, Christine, BIEBL, Manfred, BRIERS, Yves, GARCÍA, Pilar. Artilysation' of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci. _Scientific Reports_. 2016. Vol. 6, núm. 35382. [consulta: 21 de gener de 2026]. ISSN: 2045-2322. [Disponible a: https://hdl.handle.net/2445/125974]