Carregant...
Tipus de document
ArticleVersió
Versió acceptadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/147236
Optical Control of Cardiac Function with a Photoswitchable Muscarinic Agonist
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Light-triggered reversible modulation of physiological functions offers the promise of enabling on-demand spatiotemporally controlled therapeutic interventions. Optogenetics has been successfully implemented in the heart, but significant barriers to its use in the clinic remain, such as the need for genetic transfection. Herein, we present a method to modulate cardiac function with light through a photoswitchable compound and without genetic manipulation. The molecule, named PAI, was designed by introduction of a photoswitch into the molecular structure of an M2 mAChR agonist. In vitro assays revealed that PAI enables light-dependent activation of M2 mAChRs. To validate the method, we show that PAI photoisomers display different cardiac effects in a mammalian animal model, and demonstrate reversible, real-time photocontrol of cardiac function in translucent wildtype tadpoles. PAI can also effectively activate M2 receptors using two-photon excitation with near-infrared light, which overcomes the scattering and low penetration of short-wave-length illumination, and offers new opportunities for intravital imaging and control of cardiac function.
Matèries
Matèries (anglès)
Citació
Citació
RIEFOLO, Fabio, MATERA, Carlo, GARRIDO CHARLES, Aida, GOMILA JUANEDA, Alexandre, SORTINO, Rosalba, AGNETTA, Luca, CLARO IZAGUIRRE, Enrique, MASGRAU, Roser, HOLZGRABE, Ulrike, BATLLE, Montserrat, DECKER, Michael, GUASCH I CASANY, Eduard, GOROSTIZA LANGA, Pablo ignacio. Optical Control of Cardiac Function with a Photoswitchable Muscarinic Agonist. _Journal of the American Chemical Society_. 2019. Vol. 141, núm. 18, pàgs. 7628-7636. [consulta: 23 de gener de 2026]. [Disponible a: https://hdl.handle.net/2445/147236]