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2022_CurrOpi_Understanding_Bolognesi_postprint.pdf (378.15 KB)

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Accepted version

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2022-06-28

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cc by-nc-d (c) Elsevier, 2022
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/197644

Understanding and evolving prions by yeast multiplexed assays

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https://doi.org/10.1016/j.gde.2022.101941

Abstract

Yeast genetics made it possible to derive the first fundamental insights into prion composition, conformation, and propagation. Fast-forward 30 years and the same model organism is now proving an extremely powerful tool to comprehensively explore the impact of mutations in prion sequences on their function, toxicity, and physical properties. Here, we provide an overview of novel multiplexed strategies where deep mutagenesis is combined to a range of tailored selection assays in yeast, which are particularly amenable for investigating prions and prion-like sequences. By mimicking evolution in a flask, these multiplexed approaches are revealing mechanistic insights on the consequences of prion self-assembly, while also reporting on the structure prion sequences adopt in vivo.

Subject

Prions, Llevats, Mutagènesi

Subject (English)

Prions, Yeast, Mutagenesis

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Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

Citation

SEUMA, Mireia and BOLOGNESI, Benedetta. Understanding and evolving prions by yeast multiplexed assays. Current Opinion in Genetics & Development. vol.75. Vol. 101941. ISSN 1879-0380. [consulted: 8 of August of 2026]. Available at: https://hdl.handle.net/2445/197644

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