Document type
ArticleVersion
Published versionPublication date
Publication license
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/124408
3D membrane segmentation and quantification of intact thick cells using cryo soft X-ray transmission microscopy: A pilot study
Journal Title
Director/Tutor
Journal ISSN
Volume Title
Related resource
Abstract
Structural analysis of biological membranes is important for understanding cell and subcellular organelle function as well as their interaction with the surrounding environment. Imaging of whole cells in three dimension at high spatial resolution remains a significant challenge, particularly for thick cells. Cryo-transmission soft X-ray microscopy (cryo-TXM) has recently gained popularity to image, in 3D, intact thick cells (similar to 10 mu m) with details of subcellular architecture and organization in near-native state. This paper reports a new tool to segment and quantify structural changes of biological membranes in 3D from cryo-TXM images by tracking an initial 2D contour along the third axis of the microscope, through a multi-scale ridge detection followed by an active contours-based model, with a subsequent refinement along the other two axes. A quantitative metric that assesses the grayscale profiles perpendicular to the membrane surfaces is introduced and shown to be linearly related to the membrane thickness. Our methodology has been validated on synthetic phantoms using realistic microscope properties and structure dimensions, as well as on real cryo-TXM data. Results demonstrate the validity of our algorithms for cryo-TXM data analysis.
Subject
Subject (English)
Citation
Citation
CÁRDENES, Rubén, et al. 3D membrane segmentation and quantification of intact thick cells using cryo soft X-ray transmission microscopy: A pilot study. PLoS One. 2017. Vol. 12, num. 4, pags. e0174324. [consulted: 9 of June of 2026]. Available at: https://hdl.handle.net/2445/124408