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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/197708
Single-cell multi-omic analysis profiles defective genome activation and epigenetic reprogramming associated with human pre-implantation embryo arrest
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During pre-implantation stages of mammalian development, maternally stored material promotes both the erasure of the sperm and oocyte epigenetic profiles and is responsible for concomitant genome activation. Here, we have utilized single-cell methylome and transcriptome sequencing (scM&T-seq) to quantify both mRNA expression and DNA methylation in oocytes and a developmental series of human embryos at single-cell resolution. We fully characterize embryonic genome activation and maternal transcript degradation and map key epigenetic reprogramming events in developmentally high-quality embryos. By comparing these signatures with early embryos that have undergone spontaneous cleav-age-stage arrest, as determined by time-lapse imaging, we identify embryos that fail to appropriately activate their genomes or undergo epigenetic reprogramming. Our results indicate that a failure to suc-cessfully accomplish these essential milestones impedes the developmental potential of pre-implantation embryos and is likely to have important implications, similar to aneuploidy, for the success of assisted reproductive cycles.
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HERNANDEZ MORA, Jose ramon, BUHIGAS, Claudia, CLARK, Stephen, GALLEGO BONILLA, Raquel del, DASKEVICIUTE, Dagne, MONTEAGUDO SÁNCHEZ, Ana, POO LLANILLO, Maria eugenia, MEDRANO, Jose vicente, SIMÓN, Carlos, MESEGUER, Marcos, KELSEY, Gavin, MONK, David. Single-cell multi-omic analysis profiles defective genome activation and epigenetic reprogramming associated with human pre-implantation embryo arrest. _Cell Reports_. 2023. Vol. 42, núm. 2. [consulta: 20 de gener de 2026]. ISSN: 2211-1247. [Disponible a: https://hdl.handle.net/2445/197708]