Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió acceptadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/127349
Lighting up G protein-coupled purinergic receptors with engineered fluorescent ligands
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
The use of G protein-coupled receptors fluorescent ligands is undergoing continuous expansion. In line with this, fluorescent agonists and antagonists of high affinity for G protein-coupled adenosine and P2Y receptors have been shown to be useful pharmacological probe compounds. Fluorescent ligands for A1R, A2AR, and A3R (adenosine receptors) and P2Y2R, P2Y4R, P2Y6R, and P2Y14R (nucleotide receptors) have been reported. Such ligands have been successfully applied to drug discovery and to GPCR characterization by flow cytometry, fluorescence correlation spectroscopy, fluorescence microscopy, fluorescence polarization, fluorescence resonance energy transfer and scanning confocal microscopy. Here we summarize recently reported and readily available representative fluorescent ligands of purinergic receptors. In addition, we pay special attention on the use of this family of fluorescent ligands revealing two main aspects of purinergic receptor biology, namely ligand binding and receptor oligomerization.
Matèries (anglès)
Citació
Citació
CIRUELA ALFÉREZ, Francisco, FERNÁNDEZ DUEÑAS, Víctor, JACOBSON, Kenneth a.. Lighting up G protein-coupled purinergic receptors with engineered fluorescent ligands. _Neuropharmacology_. 2015. Vol. 98, núm. 58-67. [consulta: 21 de gener de 2026]. ISSN: 0028-3908. [Disponible a: https://hdl.handle.net/2445/127349]