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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/179776
Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates
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Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation.
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VENGUT CLIMENT, Empar, et al. Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates. Bioorganic & Medicinal Chemistry Letters. 2013. Vol. 23, num. 14, pags. 4048-4051. ISSN 0960-894X. [consulted: 17 of August of 2026]. Available at: https://hdl.handle.net/2445/179776