Carregant...
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/143332
A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
C-mannosylation was recently identified in the thrombospondin-related anonymous protein
(TRAP) from Plasmodium falciparum salivary gland sporozoites. A candidate P. falciparum
C-mannosyltransferase (Pf DPY-19) was demonstrated to modify thrombospondin type 1
repeat (TSR) domains in vitro, exhibiting a different acceptor specificity than their mammalian counterparts. According to the described minimal acceptor of Pf DPY19, several TSR
domain-containing proteins of P. falciparum could be C-mannosylated in vivo. However,
the relevance of this protein modification for the parasite viability remains unknown. In
the present study, we used CRISPR/Cas9 technology to generate a Pf DPY19 null mutant,
demonstrating that this glycosyltransferase is not essential for the asexual blood development
of the parasite. Pf DPY19 gene disruption was not associated with a growth phenotype, not
even under endoplasmic reticulum-stressing conditions that could impair protein folding.
The data presented in this work strongly suggest that Pf DPY19 is unlikely to play a critical
role in the asexual blood stages of the parasite, at least under in vitro conditions.
Matèries
Matèries (anglès)
Citació
Col·leccions
Citació
LÓPEZ GUTIÉRREZ, Borja, COVA, Marta, IZQUIERDO LÁZARO, Luis. A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development. _Parasitology_. 2019. [consulta: 8 de abril de 2026]. ISSN: 0031-1820. [Disponible a: https://hdl.handle.net/2445/143332]