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2008-03-28

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/177459

Kv1.5 association modifies Kv1.3 traffic and membrane localization

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Recurs relacionat

https://doi.org/10.1074/jbc.M708223200

Resum

Kv1.3 activity is determined by raft association. In addition to Kv1.3, leukocytes also express Kv1.5, and both channels control physiological responses. Because the oligomeric composition may modify the channel targeting to the membrane, we investigated heterotetrameric Kv1.3/Kv1.5 channel traffic and targeting in HEK cells. Kv1.3 and Kv1.5 generate multiple heterotetramers with differential surface expression according to the subunit composition. FRET analysis and pharmacology confirm the presence of functional hybrid channels. Raft association was evaluated by cholesterol depletion, caveolae colocalization, and lateral diffusion at the cell surface. Immunoprecipitation showed that both Kv1.3 and heteromeric channels associate with caveolar raft domains. However, homomeric Kv1.3 channels showed higher association with caveolin traffic. Moreover, FRAP analysis revealed higher mobility for hybrid Kv1.3/Kv1.5 than Kv1.3 homotetramers, suggesting that heteromers target to distinct surface microdomains. Studies with lipopolysaccharide-activated macrophages further supported that different physiological mechanisms govern Kv1.3 and Kv1.5 targeting to rafts. Our results implicate the traffic and localization of Kv1.3/Kv1.5 heteromers in the complex regulation of immune system cells.

Matèries

Membranes cel·lulars, Canals de potassi, Metabolisme

Matèries (anglès)

Cell membranes, Potassium channels, Metabolism

Citació

Col·leccions

Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citació

VICENTE GARCÍA, Rubén, VILLALONGA, Núria, CALVO ADEMUZ, Maria, ESCALADA, Artur, SOLSONA SANCHO, Carles, SOLER PRAT, Concepció, TAMKUN, Michael m., FELIPE CAMPO, Antonio. Kv1.5 association modifies Kv1.3 traffic and membrane localization. _Journal of Biological Chemistry_. 2008. Vol. 283, núm. 13, pàgs. 8756-8764. [consulta: 25 de febrer de 2026]. ISSN: 0021-9258. [Disponible a: https://hdl.handle.net/2445/177459]

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