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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/188962
Public Cord Blood Banks as a source of starting material for clinical grade HLA-homozygous induced pluripotent stem cells
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Background The increasing number of clinical trials for induced pluripotent stem cell (iPSC)-derived cell therapy products makes the production on clinical grade iPSC more and more relevant and necessary. Cord blood banks are an ideal source of young, HLA-typed and virus screened starting material to produce HLA-homozygous iPSC lines for wide immune-compatibility allogenic cell therapy approaches. The production of such clinical grade iPSC lines (haplolines) involves particular attention to all steps since donor informed consent, cell procurement and a GMP-compliant cell isolation process. Methods Homozygous cord blood units were identified and quality verified before recontacting donors for informed consent. CD34+ cells were purified from the mononuclear fraction isolated in a cell processor, by magnetic microbeads labelling and separation columns. Results We obtained a median recovery of 20.0% of the collected pre-freezing CD34+, with a final product median viability of 99.1% and median purity of 83.5% of the post-thawed purified CD34+ population. Conclusions Here we describe our own experience, from unit selection and donor reconsenting, in generating a CD34+ cell product as a starting material to produce HLA-homozygous iPSC following a cost-effective and clinical grade-compliant procedure. These CD34+ cells are the basis for the Spanish bank of haplolines envisioned to serve as a source of cell products for clinical research and therapy.
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ÁLVAREZ PALOMO, Ana belén, VEIGA, Anna, RAYA CHAMORRO, Ángel, CODINACH, Margarita, TORRENTS, Silvia, PONCE VERDUGO, Laura, RODRIGUEZ AIERBE, Clara, CUELLAR, Leopoldo, ALENDA, Raquel, ARBONA, Cristina, HERNÁNDEZ MARAVER, Dolores, FUSTÉ, Cristina, QUEROL GINER, Sergi. Public Cord Blood Banks as a source of starting material for clinical grade HLA-homozygous induced pluripotent stem cells. _Stem Cell Research & Therapy_. 2022. Vol. 13, núm. 1. [consulta: 21 de gener de 2026]. ISSN: 1757-6512. [Disponible a: https://hdl.handle.net/2445/188962]