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Impact in Plasma Metabolome as Effect of Lifestyle Intervention for Weight-Loss Reveals Metabolic Benefits in Metabolically Healthy Obese Women

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Little is known regarding metabolic benefits of weight loss (WL) on the metabolically healthy obese (MHO) patients. We aimed to examine the impact of a lifestyle weight loss (LWL) treatment on the plasma metabolomic profile in MHO individuals. Plasma samples from 57 MHO women allocated to an intensive LWL treatment group (TG, hypocaloric Mediterranean diet and regular physical activity, n = 30) or to a control group (CG, general recommendations of a healthy diet and physical activity, n = 27) were analyzed using an untargeted 1H NMR metabolomics approach at baseline, after 3 months (intervention), and 12 months (follow-up). The impact of the LWL intervention on plasma metabolome was statistically significant at 3 months but not at follow-up and included higher levels of formate and phosphocreatine and lower levels of LDL/VLDL (signals) and trimethylamine in the TG. These metabolites were also correlated with WL. Higher myo-inositol, methylguanidine, and 3-hydroxybutyrate, and lower proline, were also found in the TG; higher levels of hippurate and asparagine, and lower levels of 2-hydroxybutyrate and creatine, were associated with WL. The current findings suggest that an intensive LWL treatment, and the consequent WL, leads to an improved plasma metabolic profile in MHO women through its impact on energy, amino acid, lipoprotein, and microbial metabolism. Keywords: NMR; hypocaloric diet; metabolically healthy obesity; metabolomics; physical activity.

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ALMANZA AGUILERA, Enrique, BRUNIUS, Carl, BERNAL LÓPEZ, M. rosa, GARCIA ALOY, Mar, MADRID GAMBÍN, Francisco javier, TINAHONES, Francisco j., GÓMEZ HUELGAS, Ricardo, LANDBERG, Rikard, ANDRÉS LACUEVA, Ma. cristina. Impact in Plasma Metabolome as Effect of Lifestyle Intervention for Weight-Loss Reveals Metabolic Benefits in Metabolically Healthy Obese Women. _Journal of Proteome Research_. 2018. Vol. 17, núm. 8, pàgs. 2600-2610. [consulta: 12 de febrer de 2026]. ISSN: 1535-3893. [Disponible a: https://hdl.handle.net/2445/168917]

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