Llibres / Capítols de llibre (Biologia Cel·lular, Fisiologia i Immunologia)
URI permanent per a aquesta col·leccióhttps://hdl.handle.net/2445/32187
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- Part del llibreSILAC-based assessment of S-palmitoylated proteins in mammalian cells by metabolic labeling and click-chemistry(Elsevier, 2025-11-21) Vandekeere, Anke; Fendt, Sarah-Maria; Aznar Benitah, Salvador; Martín Pérez, MiguelS-palmitoylation of cysteine residues is the only lipid-based posttranslational modification of proteins that is reversible and therefore has important implications in cellular function. S-palmitoylation has been associated with several cellular processes (e.g., cell signaling, protein transport, cell cycle, immune response, lipid metabolism, host–pathogen interaction) and human diseases, including neurological disorders, cancer, and infectious diseases. However, S-palmitoylation research has been hampered by the cumbersome experimental protocols necessary for its study. Currently, there are two main methodologies that, coupled with mass spectrometry (MS), allow the study of S-palmitoylated proteins proteome-wide. They mainly differ in the way of labeling palmitoylated proteins: one relies on “metabolic labeling” with a palmitic acid analog in living cells, while the other is based on “chemical labeling” of thiol groups derived from palmitoylated sites in extracted proteins. Although metabolic labeling is restricted to cultured cells, we will focus on this technique as it is more sensitive and specific than others. Here, we describe the protocol to measure palmitoylation in cancer cells using metabolic labeling coupled to SILAC-based mass spectrometry quantification which can be applied to other mammalian cell models. Facilitating the use of this methodology will extend the knowledge of palmitoylation signaling and unravel potential therapeutic avenues for diseases in which this unexplored modification is implicated.
Part del llibreHuman Translational Research in Psoriasis Using CLA+ T Cells(IntechOpen, 2017-07) Ruiz Romeu, Ester; Santamaria Babí, Luis F.Focusing on the study of human memory CLA+ T cells to understand psoriasis pathology constitutes an innovative approach to explore the pathological mechanism of this chronic cutaneous inflammatory disease. CLA+ T cells can be considered peripheral cell biomarkers in the study of T-cell mediated human skin diseases. During the last few years, new evidences have been found that link streptococcal infection with IL-17 response in psoriasis by studying the interaction between Streptococcus pyogenes with CLA+ T cells and autologous epidermal cells. S. pyogenes constitutes the best clinically characterized trigger of psoriasis and by exploring its effect on CLA+ T cells and epidermal cells in psoriasis may allow understanding psoriasis by using patient’s clinical samples ex vivo.- Part del llibreResearch on Skeletal Muscle Diseases Using Pluripotent Stem Cells(IntechOpen, 2015) Oñate, Lorena de; Garreta, Elena; Tarantino, Carolina; Martínez Fraiz, Elena; Capilla Campos, Encarnación; Navarro Álvarez, Isabel; Gutiérrez Fruitós, Joaquín; Samitier i Martí, Josep; Campistol Plana, Josep M.; Muñoz-Cánovas, Pura; Montserrat Pulido, NúriaThe generation of induced pluripotent stem cells (iPSCs), especially the generation of patient-derived pluripotent stem cells (PSCs) suitable for disease modelling in vitro, opens the door for the potential translation of stem-cell related studies into the clinic. Successful replacement, or augmentation, of the function of damaged cells by patientderived differentiated stem cells would provide a novel cell-based therapy for skeletal muscle-related diseases. Since iPSCs resemble human embryonic stem cells (hESCs) in their ability to generate cells of the three germ layers, patient-specific iPSCs offer definitive solutions for the ethical and histo-incompatibility issues related to hESCs. Indeed human iPSC (hiPSC)-based autologous transplantation is heralded as the future of regenerative medicine. Interestingly, during the last years intense research has been published on disease-specific hiPSCs derivation and differentiation into relevant tissues/organs providing a unique scenario for modelling disease progression, to screen patient-specific drugs and enabling immunosupression-free cell replacement therapies. Here, we revise the most relevant findings in skeletal muscle differentiation using mouse and human PSCs. Finally and in an effort to bring iPSC technology to the daily routine of the laboratory, we provide two different protocols for the generation of patient-derived iPSCs.
- Part del llibreStructural and Functional Evolution of Glucose Transporter 4 (GLUT4): A Look at GLUT4 in Fish(IntechOpen, 2014-06-18) Marín Juez, Rubén; Capilla Campos, Encarnación; Carvalho-Simoes, Francisco; Camps Camprubí, Marta; Planas Vilarnau, JosepThe insulin-responsive glucose transporter GLUT4 was first described in 1988 as a result of studies on the regulation of glucose metabolism by insulin [1]. Soon after the discovery of GLUT4, several groups cloned GLUT4 in the human [2], rat [3,4] and mouse [5]. Since its discovery, GLUT4 has received, together with GLUT1, more experimental attention than any other single membrane transport protein. Structurally, GLUT4 follows the predicted model for class I glucose transporters. GLUT4 has a high affinity for glucose, with a Km of approximately 5 mM [6], and also transports mannose, galactose, dehydroascorbic acid and glucosamine [7-10]. In mammals, GLUT4 is mainly expressed in cardiac and skeletal muscle, brown and white adipose tissue, and brain [6,11,12]. GLUT4 plays a pivotal role in whole body glucose homeostasis, mediating the uptake of glucose regulated by insulin [13,14]. GLUT4 is responsible for the reduction in the postprandial rise in plasma glucose levels [6]. Insulin acts by stimulating the translocation of specific GLUT4-containing vesicles from intracellular stores to the plasma membrane (PM) resulting in an immediate increase in glucose transport [6,15]. The disruption of GLUT4 expression has been extensively associated with pathologies of impaired glucose uptake and insulin resistance such as type 2 diabetes and obesity [13,16-18]...
- Part del llibreNeuro-EPO by Nasal Route as a Neuroprotective Therapy in Brain Ischemia(IntechOpen, 2012-01-18) García Rodríguez, Julio César; Rama Bretón, RamónCerebral vascular diseases (CVD) are the third leading cause of death in industrialized countries and in Cuba affect the 50% of the population above 60 years old. The mortality is exponentially increased with age and doubles every five years. A total of 22,000 annual cases are estimated in Cuba, a country where life expectance should increase to 80 years in the near future [1]. CVD are often followed by a high social and individual cost as a consequence of disability and family affectation. The most problematic among such diseases is ischemic cerebral vascular disease, characterized by the reduction of cerebral blood flow (CBF) below a critical level. From this initial event several processes take place to induce the clinical symptoms of cerebral ischemia...
- Part del llibreExcitotoxicity and Oxidative Stress in Acute Ischemic Stroke(IntechOpen, 2012-01-18) Rama Bretón, Ramón; García Rodríguez, Julio CésarThe term “stroke” is applied to a heterogeneous group of diseases caused by decreased perfusion of the brain due to occlusion of the blood vessels supplying the brain or a haemorrhage originating in them. Most strokes (~ 85%) are ischemic; that is, they result from occlusion of a major cerebral artery by a thrombus or embolism. This results in reduced blood flow and a major decrease in the supply of oxygen and nutrients to the affected region. The rest of strokes are haemorrhagic: caused by the rupture of a blood vessel either in the brain or on its surface. Strokes deprive the brain not only of oxygen but also of glucose and of all other nutrients, as well as disrupting the nutrient/waste exchange process required to support brain metabolism. The result is the development of a hypoxic-ischemic state. Ischemia is defined as a decrease in blood flow to tissues that prevents adequate delivery of oxygen, glucose and others nutrients. Ischemic stroke is the result of total or partial interruption of cerebral arterial blood supply, which leads to oxygen and glucose deprivation of the tissue (ischemia). If cerebral arterial blood flow is not restored within a short period, cerebral ischemia is the usual result, with subsequent neuron death within the perfusion territory of the vessels affected. Ischemic stroke is characterized by a complex sequence of events that evolves over hours or even days [1-3]. Acute ischemic stroke results from acute occlusion of cerebral arteries. Cerebral ischemia occurs when blood flow to the brain decreases to a level where the metabolic needs of the tissue are not met. Cerebral ischemia may be either transient (followed by reperfusion) or essentially permanent. In all cases, a stroke involves dysfunction and death of brain neurons and neurological damage that reflects the location and size of the brain area affected.
- Part del llibre¿Qué hacen después del grado los Egresados y Egresadas de los Grados de Biotecnología y de Bioquímica de a Universitat de Barcelona?(Servicio Editorial de la Universidad del País Vasco, 2020-02) Reina del Pozo, Manuel; Müller Sánchez, Claudia Alejandra; Campos Bonilla, Begoña[spa] Con frecuencia nos han hecho, como profesores, la pregunta “¿qué puedo hacer después de terminar el grado/master/doctorado?”, y como profesores hemos respondido con la mejor voluntad en función de nuestra experiencia. Este estudio surge de la curiosidad por la trayectoria profesional que hayan podido seguir los egresados de los Grados de Bioquímica y Biotecnología de la Universitat de Barcelona, y por el interés en poder dar una respuesta más amplia, completa y documentada a esa pregunta. Se ha basado en la recogida de información mediante encuesta de las trayectorias profesionales de 393 egresados de los 451 (87,1%) que se graduaron entre 2012 y 2016. Se usaron principalmente preguntas abiertas, en las que se pedía a los egresados que explicaran lo que hacían en ese momento, y cómo habían llegado a ello. Los resultados presentaron varias sorpresas. La primera es que más del 80% de los egresados dicen dedicarse profesionalmente a actividades relacionadas con lo que han estudiado, de que alrededor del 80% de los egresados cursan estudios de postgrado, con un 23% de ellos de tipo industrial y profesionalizadores, y que alrededor del 40% de los egresados cursan un doctorado. No ha sido un estudio ni fácil ni rápido, pero una vez realizado el sondeo y el análisis de toda la información pensamos que aporta una visión muy rica de lo que los egresados que salen de nuestras aulas son capaces de hacer.
- Part del llibreLa docencia práctica de cultivos celulares en los grados de Bioquímica y Biotecnología de la Universitat de Barcelona(Servicio Editorial de la Universidad del País Vasco, 2020-02) Reina del Pozo, Manuel; Martínez Estrada, Ofelia María; Soriano Zaragoza, Francesc X. (Francesc Xavier); Müller Sánchez, Claudia Alejandra; Casellas Díaz, Sergi; Torres Cano, Alejo; García Melero, Ana; Ramiro-Pareta, Marina; Segarra Mondéjar, Marc; Campos Bonilla, BegoñaLas técnicas de cultivo celular son de gran importancia tanto en la investigación científica (bioquímica, biotecnológica, biomédica...) como en numerosas aplicaciones industriales entre las que destacaríamos la producción de biofármacos y la medicina regenerativa. En los Grados de Bioquímica y de Biotecnología impartidos en la Facultad de Biología de la Universitat de Barcelona esta competencia se trabaja especialmente en una asignatura, ‘Cultivos Celulares e Ingeniería Tisular’, en el sexto semestre. El reto de esta docencia es la incorporación de prácticas adecuadas para que el alumno, al final de las mismas, conozca los procedimientos básicos y alguna de sus aplicaciones. Se han diseñado, implementado e impartido prácticas de cultivo celular animal durante 8 cursos a un total de 1047 alumnos con una gran aceptación. Las sesiones prácticas en el laboratorio son precedidas por una explicación de las mismas en el aula y la elaboración de esquemas de los procedimientos. En el laboratorio, bien equipado para el cultivo celular, los alumnos trabajan en grupos de 20 alumnos con dos profesores, formando equipos de 4 a 5 alumnos que son independientes, durante 5-6 horas de lunes a viernes, realizando un total de 4 experimentos. La evaluación de esta actividad se basa en la evaluación del desempeño del alumno en el laboratorio, así como la valoración de un informe de las prácticas, que se devuelve al alumno corregido y comentado. El alumno valora estas prácticas de manera muy positiva. El principal indicador de su valor es el hecho de que en más del 60% de los trabajos finales de Grado realizados utilizan técnicas de cultivo celular. Por ello manifiestan su satisfacción al sentirse familiarizados con técnicas y equipos de cultivo celular al iniciar su trabajo de investigación.
- Objecte de conferènciaStrategies against β-amyloid protein as therapeutics in Alzheimer's disease(Research Signpost, 2017) Folch, Jaume; Ettcheto Arriola, Miren; Busquets Figueras, Oriol; Sánchez-López, E. (Elena); Castro-Torres, Rubén Darío; Beas Zárate, Carlos; Pallàs i Llibería, Mercè, 1964-; Olloquequi, Jordi; Jara, Daniela; García López, María Luisa; Auladell i Costa, M. Carme; Camins Espuny, Antoni
- LlibreAdvances in comparative endocrinology : vol. VII(Edicions de la Universitat de Barcelona i Asociación Ibérica de Endocrinologia Comparada, 2015) Asociación Ibérica de Endocrinología ComparadaThe present volume of the series Advances in Comparative Endocrinology contains the contributions of the participants of the 9th Congress of the “Asociación Ibérica de Endocrinología Comparada” (AIEC) that took place at the University of Barcelona in July 2013. This volume includes a great variety of studies of prestigious and international recognized research groups using vertebrate and invertebrate species, contributing with the most recent insights related to the hormonal control of important physiological processes such us growth, development, food intake, metabolism, stress and reproduction among others.
- AltresAtles d’histopatologia de mol·luscs bivalves. Introducció a la histologia d'algunes espècies de mol·luscs bivalves marins i les seves parasitosis més freqüents(2015-11) Bozzo, Maria Gracia; Durfort i Coll, Mercè; Poquet Miquel, Montserrat; Sagristà i Mateo, Elena; Universitat de Barcelona. Departament de Biologia Cel·lular[cat] Els exemplars de mol·luscs bivalves estudiats procedeixen principalment del Delta de l´Ebre (badia dels Alfacs i del Fangar) i les ostres han estat de dues procedències diferents: Delta de l´Ebre i de Sète (França). S´han estudiat individus immadurs (juvenils) i s´ha seguit el procés de maduració sexual en tots els casos. Les tècniques aplicades en aquest estudi han seguit els protocols estàndards. Per la Microscòpia Òptica: fixació en formol al 10% o en Bouin. Inclusió en parafina i obtenció de talls de cinc a set micres per terme mig. Les tincions rutinàries han estat post vitals amb hematoxilina-eosina (Hem-Eos) y també amb el tricròmic de Mallory. El taronja d´acridina s´ha emprat per les observacions amb el microscopi de fluorescència i el iodur de propidi-fal·loïdina-TRITC (isocianat de tetrametilrodamina) per les observacions amb el microscopi confocal i en ocasions també s´ha utilitzat la doble tinció hematoxilina-eosina (Hem-Eos). En alguns casos s´han fet tincions vitals amb roig neutre i amb blau de metilè. Per l´estudi amb la Microscòpia Electrònica de Rastreig (MER) la fixació ha estat en glutaraldehid-paraformaldehid preparat amb tampó de Sörensen o amb cacodilat i l´ombrejat amb carbó i també s´ha metal·litzat amb or o amb una aleació d´or-pal·ladi. Per la Microscòpia Electrònica de Transmissió (MET) la fixació ha estat doble: glutaraldehid-paraformaldehid preparat amb la solució de Sörensen (a base de fosfats) o amb cacodilat al 0,1 M seguit d´una postfixació, generalment amb tetraòxid d´osmi a l´1% igualment tamponat amb la mateixa solució emprada en la primera fixació. La inclusió ha estat preferentment feta amb Spurr i els talls semifins (de control) tenyits amb blau de metilè-bòrax i els ultrafins contrastats amb acetat d´uranil i citrat de plom. Les observacions s´han fet amb microscopis electrònics Leica, Hitachi, Jeol, Philips y Zeiss dels Centres Científics i Tecnològics de la Universitat de Barcelona.
- Part del llibreRole of JNK in neurodegenerative diseases(Transworld Research Network, 2012) Junyent Herena, Fèlix; Verdaguer Cardona, Ester; Folch, Jaume; Beas Zárate, Carlos; Pallàs i Llibería, Mercè, 1964-; Auladell i Costa, M. Carme; Camins Espuny, AntoniThe c-Jun N-terminal kinases (JNK) are members of the MAPK family and can be activated by different stimuli such as cellular stress, heat shock and ultra-violet irradiation. JNKs have different physiological functions and they have been linked to apoptosis in different cell types. Therefore, the JNK signalling pathway is an important target to prevent cell death. In the present chapter, the role of JNKs in neurodegenerative diseases will be discussed, as well as the pharmacological compounds that inhibit this signalling pathway as therapeutic intervention to prevent neuronal death.