Dades (Bioquímica i Biomedicina Molecular)
URI permanent per a aquesta col·leccióhttps://diposit.ub.edu/handle/2445/69232
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AltresEffect of sex on glucose handling by adipocytes isolated from rat subcutaneous, mesenteric and perigonadal adipose tissue (Raw data)(2018-01) Rotondo, Floriana; Ho-Palma, Ana Cecilia; Remesar Betlloch, Xavier; Fernández López, José Antonio; Romero Romero, María del Mar; Alemany, Marià, 1946-- AltresIn Rats Fed High-Energy Diets, Taste –Rather than Fat Content– Is the Key Factor Increasing Food Intake. Comparison of a Cafeteria and a Lipid-Supplemented Standard Diet (Raw data)(2017-05-16) Oliva Lorenzo, Laia; Aranda, Tània; Caviola, Giada; Fernández-Bernal, Anna; Alemany, Marià, 1946-; Fernández López, José Antonio; Remesar Betlloch, XavierBackground: Diet deeply affects the food selection and ingestion both in humans and rodents, often resulting in excess energy intake. Methods: We investigated this process comparing two different high-fat dietary approaches to induce obesity, in which all rats received about 40% of their energy intake as lipids. The main nutrient difference between the diets, when compared with controls fed standard lab chow, was the lipid content. Cafeteria diets (K) were devised to be tasty, and thus highly desirable to the rats, mainly for its diverse mix of tastes, particularly salty and sweet. This diet was compared with another high-fat (HF) potentially obesogenic diet, devised not to be as tasty as K, and prepared just supplementing standard chow pellets with fat. We also analysed the influence of sex on the effects of the diets. Results: K rats grew faster, especially the males, although females showed a higher proportion of body lipid, because of a high lipid, sugar and protein intake. HF weight change rates were not different from those of controls. In addition to high sugar, K rats also ingested large amounts of salt. With this study we have shown that the key factor eliciting the excess energy intake in a high-energy diet rat model was not solely or mainly their fat intake. The changes in body fat accrual were more a consequence of their appetence for the food. Conclusions: The results show that the significant presence of sugar and salt is a powerful factor promoting excess food intake, more effective than increasing diet lipid content. These effects were already observed after a relatively short treatment, additionally confirming the differential effects of sex on the hedonic and obesogenic response to diet.
- AltresQuantitative analysis of rat adipose tissue cell recovery, and non-fat cell volume, in primary cell cultures (Raw data)(2016-09-29) Rotondo, Floriana; Romero Romero, María del Mar; Ho-Palma, Ana Cecilia; Remesar Betlloch, Xavier; Fernández López, José Antonio; Alemany, Marià, 1946-Background. White adipose tissue (WAT) is a complex, diffuse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defence, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT by breaking up the matrix of collagen fibres, however, it is unclear to what extent adipocyte number in primary cultures correlates with their number in intact WAT, since recovery and viability are often unknown. Experimental design. Epididymal WAT of 4-6 young adult rats was used to isolate adipocytes with collagenase. Careful recording of lipid content of tissue, and all fraction volumes and weights, allowed us to trace the amount of initial WAT fat remaining in the cell preparation. Functionality was estimated by incubation with glucose and measurement of lactate, glycerol and NEFA production. Non-adipocyte cells were also recovered and their sizes (and those of adipocytes) were also measured. The presence of non-nucleated cells (erythrocytes) was also estimated. Results. Cell numbers and sizes were correlated from all fractions to intact WAT. Tracing the lipid content, the recovery of adipocytes in the final, metabolically active, preparation was in the range of 70-75%. Adipocytes were 7%, erythrocytes 68% and other stromal (nucleated cells) 24% of total WAT cells. However, their overall volumes were, 91%, 0.05%, and 0.2% of WAT. Non-fat volume of adipocytes was 2.5% of WAT. Conclusions. The methodology presented here allows for a direct quantitative reference to the original tissue of studies using isolated cells. We have found, also, that the "live cell mass" of adipose tissue is very small (about 25 µL/g for adipocytes and 2 µL/g stromal, plus about 1 µL/g blood). This fact, translates (with respect to the actual "live cytoplasm" size) into an extremely high metabolic activity, which make WAT an even more significant agent in the control of energy metabolism.
- AltresGuia de medicaments d’Atenció Primària que requereixen una vigilància especial per la seva dispensació en pacients amb funció renal disminuïda.(2016-01) March Pujol, Marian; Via Sosa, Ma. de los Ángeles; Rubio Valera, Maria; Roig Martínez, Marta; Travé i Mercadé, Pere; Martínez Castelao, Alberto; Gorris Teruel, José Luis; Alcázar, Roberto (Alcázar Arroyo); Martínez Fernández, IsabelAquesta Base de Dades (Guia de Medicaments) recull informació per tal d’indicar quins medicaments són susceptibles d'ajustos de dosi per evitar la iatrogènia medicamentosa en pacients amb deteriorament de la funció renal. Aquesta informació s'ha consensuat entre farmacèutics i metges nefròlegs a partir de la informació disponible en diferents bases de dades nacionals i internacionals. Per agilitzar l'ús de la Guia s'ha consensuat categoritzar en nivell baix, moderat o alt el risc que suposa pel pacient l’ús d'aquests medicaments segons el seu filtrat glomerular. A més la Guia recull els ajustos de dosi a realitzar, les interaccions medicamentoses i la simptomatologia per sobre dosificació en pacients amb funció renal disminuïda. A partir d'aquesta Base de Dades, s'ha dissenyat una aplicació web que facilita al professional sanitari la presa de decisions per a l'ajust de dosis de medicaments en funció del filtrat glomerular del pacient.
- AltresEffects of sex and site on amino acid metabolism enzymes gene expression and activity in rat white adipose tissue (Raw Data)(2015-09-14) Arriarán, Sofía; Agnelli, Silvia; Remesar Betlloch, Xavier; Fernández López, José Antonio; Alemany, Marià, 1946-Dades experimentals a partir de les quals s'han calculat activitats enzimàtiques i expressions d'enzims (i altres proteïnes) relacionats amb el cicle de la urea en diferents localitzacions (masses) de teixit adipós blanc de rates (mascles i femelles). Els arxius contenen les dades experimentals directes i els paràmetres de control i de referència, derivats dels animals, que han estat processats per a la publicació. També inclouen algunes fases del processament de les dades per assolir els resultats finals, de forma que el conjunt es pugui comprendre globalment.