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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/182623
Acute RyR1 Ca2+ leak enhances NADH-linked mitochondrial respiratory capacity
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Sustained ryanodine receptor (RyR) Ca2+ leak is associated with pathological conditions such as heart failure or skeletal muscle weakness. We report that a single session of sprint interval training (SIT), but not of moderate intensity continuous training (MICT), triggers RyR1 protein oxidation and nitrosylation leading to calstabin1 dissociation in healthy human muscle and in in vitro SIT models (simulated SIT or S-SIT). This is accompanied by decreased sarcoplasmic reticulum Ca2+ content, increased levels of mitochondrial oxidative phosphorylation proteins, supercomplex formation and enhanced NADH-linked mitochondrial respiratory capacity. Mechanistically, (S-)SIT increases mitochondrial Ca2+ uptake in mouse myotubes and muscle fibres, and decreases pyruvate dehydrogenase phosphorylation in human muscle and mouse myotubes. Countering Ca2+ leak or preventing mitochondrial Ca2+ uptake blunts S-SIT-induced adaptations, a result supported by proteomic analyses. Here we show that triggering acute transient Ca2+ leak through RyR1 in healthy muscle may contribute to the multiple health promoting benefits of exercise.
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ZANOU, Nadège, DRIDI, Haikel, REIKEN, Steven, IMAMURA DE LIMA, Tanes, DONNELLY, Chris, DE MARCHI, Umberto, FERRINI, Manuele, VIDAL, Jeremy, SITTENFELD, Leah, FEIGE, Jerome n., GARCÍA-ROVES, Pablo m. (pablo miguel), LOPEZ-MEJIA, Isabel c., MARKS, Andrew r., AUWERX, Johan, KAYSER, Bengt, PLACE, Nicolas. Acute RyR1 Ca2+ leak enhances NADH-linked mitochondrial respiratory capacity. _Nature Communications_. 2021. Vol. 12, núm. 1, pàgs. 7219. [consulta: 20 de gener de 2026]. ISSN: 2041-1723. [Disponible a: https://hdl.handle.net/2445/182623]