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2010

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/134028

Development and validation of a high performance liquid chromatography method to determine linezolid concentrations in pig pulmonary tissue

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Recurs relacionat

https://doi.org/10.1515/CCLM.2010.078

Resum

Background: linezolid is the first synthetic compound of a new group of antimicrobials, the oxazolidinones, which inhibit protein synthesis. It shows a broad spectrum of activity against Gram positive organisms. With respect to its pharmacokinetics, linezolid shows a relatively high volume of distribution and good penetration into inflammatory fluids, bone, fat and muscle. Methods: a reversed-phase isocratic high-performance liquid chromatographic method for linezolid analysis in piglet pulmonary tissue is described. Tissue samples and controls were prepared in 1 x TBE (1 M Tris, 0.9 M boric acid, 0.01 M EDTA). The mobile phase consisted of 20% ultrafiltered water and 80% of (A) 15 mM potassium monohydrogen phosphate buffer (pH = 5) with (B) acetonitrile (80%/20%; v/v). Samples were homogenized and precipitated with HClO(4) 3% (1/1, v/v). The injection volume was 100 microL. Ofloxacin was used as an internal standard. Results: the assay was linear over a linezolid concentration range: 1.6-100 microg/mL. The method provided good validation data (n = 15): inaccuracy (3.6%), intra and inter-day variability (4.2% and 5.2%, respectively), recovery (91.8%), limit of detection (0.8 microg/mL) and quantitation (1.6 microg/mL) and acceptable stability within 24 h in the auto-sampler. Conclusions: the method offers a fast and simple approach to determine linezolid in pulmonary tissue which could be of use in pharmacokinetic studies.

Matèries

Anàlisi, Agents antiinfecciosos, Cromatografia de líquids d'alta resolució, Pulmó, Química, Compostos heterocíclics

Matèries (anglès)

Assaying, Anti-infective agents, High performance liquid chromatography, Lung, Chemistry, Heterocyclic compounds

Citació

Col·leccions

Articles publicats en revistes (Medicina)
Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Articles publicats en revistes (Infermeria de Salut Pública, Salut mental i Maternoinfantil)

Citació

GUERRERO MOLINA, Laura, et al. Development and validation of a high performance liquid chromatography method to determine linezolid concentrations in pig pulmonary tissue. Clinical Chemistry and Laboratory Medicine. 2010. Vol. 3, num. 48, pags. 391-398. ISSN 1434-6621. [consulted: 12 of August of 2026]. Available at: https://hdl.handle.net/2445/134028

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