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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/171756
Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates
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The human commensal Haemophilus parainfluenzae is emerging as an opportunistic multidrug-resistant pathogen. The objectives of this work were to characterise a new capsular operon of extensively drug-resistant (XDR) H. parainfluenzae clinical isolates and study their resistance mechanisms using whole-genome sequencing. All strains were resistant to: ß-lactams, via amino acid changes in PBP3 (S385T, I442F, V511A, N526K and V562I); quinolones, by alterations in GyrA (S84F and D88Y) and ParC (S84F and S138T); chloramphenicol, through the presence of catS; macrolides, via the presence of mel and mef(E)-carrying MEGA element; and tetracycline, through the presence of tet(M) and/or tet(B). Phylogenetic analysis revealed high genomic diversity when compared to the H. parainfluenzae genomes available on the NCBI, the isolates from this study being closely related to the Swiss XDR AE-2096513. A full capsular operon showing homology to that of H. influenzae was identified, in accordance with the observation of a capsular structure by TEM. This study describes for the first time a capsular operon in H. parainfluenzae, a major determinant of pathogenicity that may contribute to increased virulence in XDR clinical isolates. Moreover, phylogenetic analysis suggests the possible spread of an XDR-encapsulated strain in Europe.
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GONZÁLEZ DÍAZ, Aida, TUBAU, Fe, PINTO, Miguel, SIERRA URUEÑA, Yanik, CUBERO, Meritxell, CÁMARA, Jordi, AYATS, Josefina, BAJANCA-LAVADO, Paula, ARDANUY TISAIRE, María carmen, MARTI, Sara. Identification of polysaccharide capsules among extensively drug-resistant genitourinary Haemophilus parainfluenzae isolates. _Scientific Reports_. 2019. Vol. 9, núm. 1, pàgs. 4481. [consulta: 24 de gener de 2026]. ISSN: 2045-2322. [Disponible a: https://hdl.handle.net/2445/171756]