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2002-03
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/135597

Pig liver carnitine palmitoyltransferase. Chimera studies show that both the N- and C-terminal regions of the enzyme are important for the unusual high malonyl-CoA sensitivity

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Pig and rat liver carnitine palmitoyltransferase I (L-CPTI) share common K(m) values for palmitoyl-CoA and carnitine. However, they differ widely in their sensitivity to malonyl-CoA inhibition. Thus, pig l-CPTI has an IC(50) for malonyl-CoA of 141 nm, while that of rat L-CPTI is 2 microm. Using chimeras between rat L-CPTI and pig L-CPTI, we show that the entire C-terminal region behaves as a single domain, which dictates the overall malonyl-CoA sensitivity of this enzyme. The degree of malonyl-CoA sensitivity is determined by the structure adopted by this domain. Using deletion mutation analysis, we show that malonyl-CoA sensitivity also depends on the interaction of this single domain with the first 18 N-terminal amino acid residues. We conclude that pig and rat L-CPTI have different malonyl-CoA sensitivity, because the first 18 N-terminal amino acid residues interact differently with the C-terminal domain. This is the first study that describes how interactions between the C- and N-terminal regions can determine the malonyl-CoA sensitivity of L-CPTI enzymes using active C-terminal chimeras.

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Matèries

Carnitina palmitoïl-transferasa 1, Cinètica enzimàtica, Aminoàcids, Mutagènesi

Matèries (anglès)

Carnitine palmitoyltransferase I, Enzyme kinetics, Amino acids, Mutagenesis

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Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

Citació

NICOT, Carine, RELAT PARDO, Joana, WOLDEGIORGIS, Gebre, HARO BAUTISTA, Diego, MARRERO GONZÁLEZ, Pedro f.. Pig liver carnitine palmitoyltransferase. Chimera studies show that both the N- and C-terminal regions of the enzyme are important for the unusual high malonyl-CoA sensitivity. _Journal of Biological Chemistry_. 2002. Vol. 277, núm. 12, pàgs. 10044-10049. [consulta: 30 de novembre de 2025]. ISSN: 0021-9258. [Disponible a: https://hdl.handle.net/2445/135597]

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