Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

602283.pdf (964.94 KB)

Document type

Article

Version

Accepted version

Publication date

2012-03-28

All rights reserved

Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/163538

Critical evaluation of the 2D-CSIA scheme for distinguishing fuel oxygenate degradation reaction mechanisms

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

https://doi.org/10.1021/es2036543

Abstract

Although the uniform initial hydroxylation of methyl tert-butyl ether (MTBE) and other oxygenates during aerobic biodegradation has already been proven by molecular tools, variations in carbon and hydrogen enrichment factors (εC and εH) have still been associated with different reaction mechanisms (McKelvie et al. Environ. Sci. Technol. 2009, 43, 2793-2799). Here, we present new laboratory-derived εC and εH data on the initial degradation mechanisms of MTBE, ethyl tert-butyl ether (ETBE) and tert-amyl methyl ether (TAME) by chemical oxidation (permanganate, Fenton reagents), acid hydrolysis and aerobic bacteria cultures (species of Aquincola, Methylibium, Gordonia, Mycobacterium, Pseudomonas and Rhodococcus). Plotting of Δδ2H/ Δδ13C data from chemical oxidation and hydrolysis of ethers resulted in slopes (Λ values) of 22 ± 4 and between 6 and 12, respectively. With A. tertiaricarbonis L108, R. zopfii IFP 2005 and Gordonia sp. IFP 2009, εC was low (<|-1|¿) and εH insignificant. Fractionation obtained with P. putida GPo1 was similar to acid hydrolysis and M. austroafricanum JOB5 and R. ruber DSM 7511 displayed Λ values previously only ascribed to anaerobic attack. The fractionation patterns rather correlate with the employment of different P450, AlkB and other monooxygenases, likely catalyzing ether hydroxylation via different transition states. Our data questions the value of 2D-CSIA for a simple distinguishing of oxygenate biotransformation mechanisms, therefore caution and complementary tools are needed for proper interpretation of groundwater plumes at field sites.

Subject

Degradació dels sòls, Hidrogen, Èters, Carburants

Subject (English)

Soil degradation, Hydrogen, Ethers, Motor fuels

Citation

Collections

Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)
Publicacions de projectes de recerca finançats per la UE

Citation

ROSELL, Mònica, et al. Critical evaluation of the 2D-CSIA scheme for distinguishing fuel oxygenate degradation reaction mechanisms. Environmental Science & Technology. 2012. Vol. 46, num. 9, pags. 4757-4766. ISSN 0013-936X. [consulted: 14 of August of 2026]. Available at: https://hdl.handle.net/2445/163538

Export metadata

JSON - METS

Share record