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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/173364
Photoswitchable dynasore analogs to control endocytosis with light
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The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single- wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration.
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Articles publicats en revistes (Química Inorgànica i Orgànica)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))
Articles publicats en revistes (Patologia i Terapèutica Experimental)
Citation
CAMARERO, Núria, et al. Photoswitchable dynasore analogs to control endocytosis with light. Chemical Science. 2020. Vol. 11, num. 8981-8988. ISSN 2041-6520. [consulted: 16 of October of 2026]. Available at: https://hdl.handle.net/2445/173364