Histological organisation of the rat duodenum
| dc.contributor.author | Pérez Clausell, Jeús | |
| dc.contributor.author | Universitat de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia | |
| dc.date.accessioned | 2025-10-08T12:00:14Z | |
| dc.date.available | 2025-10-08T12:00:14Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | [ENG] A collection of micrographs of the duodenum in rats and mice. General views of the intestinal wall and detailed images of each layer are shown: mucosa – comprising the epithelium, lamina propria and muscularis mucosæ –, submucosa, muscularis externa and serosa. Specific structures such as the brush border, goblet cells, crypts, Brunner's glands, and nerve ganglia – both submucosal and myenteric – are also illustrated. Paraffin sections were stained with hæmatoxylin-eosin or van Gieson's trichrome, while epoxy semi-thin sections (2 µm thick) were stained with toluidine blue. This collection complements the micrographs published by Dr Elena Sagristà i Mateo → https://hdl.handle.net/2445/60214 ← See also: → https://diposit.ub.edu/dspace/browse?type=author&value=Sagrist%C3%A0+i+Mateo%2C+Elena ← | ca |
| dc.description.abstract | [CAT] Col·lecció de micrografies de l'organització del duodè de la rata i ratolí. S'inclouen imatges de la paret intestinal i detalls de cada capa: mucosa (que inclou l'epiteli, làmina propria, muscularis mucosæ), submucosa, muscularis externa i serosa. També es mostren detalls de l'epiteli en raspall, les cèl·lules caliciformes, les criptes, les glàndules de Brunner i els ganglis dels plexes submucós i mientèric. S'han utilitzat talls de parafina tenyits amb hematoxilina-eosina o el tricròmic de van Gieson, i talls semifins de 2 µm tenyits amb blau de toluïdina. Aquesta col·lecció complementa les micrografies publicades per la Dra. Elena Sagristà i Mateo → https://hdl.handle.net/2445/60214 ← See also: → https://diposit.ub.edu/dspace/browse?type=author&value=Sagrist%C3%A0+i+Mateo%2C+Elena ← | ca |
| dc.format.extent | 88 imatges | |
| dc.format.mimetype | image/jpeg | |
| dc.identifier.citation | Cite as: Pérez-Clausell J (2025) << Title>>. Universitat de Barcelona, Dipòsit digital [consulted on <<date>>] https://hdl.handle.net/2445/?????? | ca |
| dc.identifier.uri | https://hdl.handle.net/2445/223556 | |
| dc.language.iso | eng | ca |
| dc.rights | cc by-nc-nd (c) Pérez Clausell, Jeús, 2025 | |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/es/ | * |
| dc.source | OMADO (Objectes i MAterials DOcents) | |
| dc.subject.classification | Histologia | cat |
| dc.subject.classification | Duodè | cat |
| dc.subject.classification | Rates (Animals de laboratori) | cat |
| dc.subject.classification | Imatges | |
| dc.title | Histological organisation of the rat duodenum | ca |
| dc.title.alternative | Organització histològica del duodè de la rata. | ca |
| dc.type | info:eu-repo/semantics/other | ca |
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- Wall of the small intestine in mice. Folds of the mucosa form finger-shaped projections termed villi (black arrowheads). These consist of a thin epithelium (ep), stained pink, and loose connective tissue within: the lightly stained lamina propria (lp). The epithelium invaginates into the wall, forming pit-like structures: the crypts. The muscularis mucosæ (mm), the deepest layer of the mucosa, is a thin strip of smooth muscle cells, and is just perceptible at this magnification. Beneath the muscularis mucosæ lies a thin submucosa (sub) and the muscularis externa (me). In the lumen of the intestine, partially digested food is visible, including portions of vegetables with polygonal cell walls (asterisks). Paraffin section, hæmatoxylin-eosin stain. v, blood vessels.
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- P_intestine_Heosin_ 4x_01.jpg
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- Wall of the small intestine in mice. No labelling.
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- P_intestine_Heosin_6_3x_03_04_collage_LETTERS.jpg
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- Wall of the small intestine in mice in oblique section (collage of micrographs allowing moderate zoom). The mucosa forms outward projections known as villi, which are lined with a simple (mono-layered) columnar epithelium (ep) stained darkly. Although the cytoplasm in most cells stains dark pink, goblet cells (Go) are distributed throughout the epithelium and contain round accumulations of mucous secretory granules that stain bluish-grey (white arrowheads). The epithelium extends into the wall, forming crypts that reach deep portions of the mucosa. The core of the villi and the space between the crypts is occupied by loose connective tissue – the lamina propria (lp) – which is palely stained. The inner or deeper border of the mucosa is marked by a thin muscularis mucosæ (mm), and beneath it lies a denser connective tissue – the submucosa (sub) –, which stains light pink but darker than the lamina propria. Both lamina propria and submucosa are profusely supplied by blood vessels (v). Situated below is the muscularis externa (me), a layer of smooth muscle cells divided into two sublayers, although not distinguishable at this magnification. Paraffin section, hæmatoxylin-eosin stain.
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- P_intestine_Heosin_6_3x_03_04_collage.jpg
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- 786.13 KB
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- Joint Photographic Experts Group/JPEG File Interchange Format (JFIF)
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- Wall of the small intestine in mice in oblique section (collage of micrographs). No labelling.
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- P_O_duodenum2_HEosin_20X_63_66_collage_LETTERS.jpg
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- 1.29 MB
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- Longitudinal section of the duodenum in a rat (collage of micrographs allowing moderate zoom). Three villi are shown lined with a simple epithelium (ep) that also invaginates into the lightly stained lamina propria (lp), forming long crypts. The lamina propria is limited internally by a thin layer of smooth muscle cells – the muscularis mucosæ (mm) – that stains pink. Underneath, the submucosa (sub) is expanded, containing numerous secretory glands (g) – Brunner's glands – with dark purple cell nuclei and pale cytoplasm. Numerous blood vessels (v) are seen here, as in the lamina propria. Deeper, the muscularis externa can be seen divided into two distinct layers: an inner or circular layer (ic) and an outer or longitudinal layer (ol). Since this is a longitudinal section of the duodenum, muscle cell nuclei appear round in the circular layer (transversely sectioned) and elongated in the longitudinal layer. A thin serosa (ser) envelopes the intestine. Paraffin section, hæmatoxylin-eosin stain. Go, goblet cells.