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2015-01

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/117127

Atomic force microscopy visualization of injuries in Enterococcus faecalis surface caused by Er,Cr:YSGG and diode lasers

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https://doi.org/10.4317/medoral.19991

Resum

Aim: To visualize by atomic force microscopy the alterations induced on Enterococcus. faecalis surface after treatment with 2 types of laser: Erbium chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser and Diode laser. Material and methods: Bacterial suspensions from overnight cultures of E. faecalis were irradiated during 30 seconds with the laser-lights at 1 W and 2 W of power, leaving one untreated sample as control. Surface alterations on treated E. faecalis were visualized by atomic force microscopy (AFM) and its surface roughness determined. Results: AFM imaging showed that at high potency of laser both cell morphology and surface roughness resulted altered, and that several cell lysis signs were easily visualized. Surface roughness clearly increase after the treatment with Er,Cr:YSGG at 2W of power, while the other treatments gave similar values of surface roughness. The effect of lasers on bacterial surfaces visualized by AFM revealed drastic alterations. Conclusions: AFM is a good tool to evaluate surface injuries after laser treatment; and could constitute a measure of antimicrobial effect that can complete data obtained by determination of microbial viability.

Matèries

Microscòpia de força atòmica, Làsers, Bacteris

Matèries (anglès)

Atomic force microscopy, Lasers, Bacteria

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Articles publicats en revistes (Odontoestomatologia)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
Articles publicats en revistes (Patologia i Terapèutica Experimental)

Citació

LÓPEZ JIMÉNEZ, Lídia, ARNABAT DOMÍNGUEZ, Josep, VIÑAS, Miquel, VINUESA AUMEDES, Teresa. Atomic force microscopy visualization of injuries in Enterococcus faecalis surface caused by Er,Cr:YSGG and diode lasers. _Medicina Oral_. Patología Oral y Cirugia Bucal. Vol.  2015, núm. 20, pàgs. 1. [consulta: 20 de gener de 2026]. ISSN: 1698-4447. [Disponible a: https://hdl.handle.net/2445/117127]

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