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2024-10-01

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cc by-nc-nd (c) Belkadi, Roumaissa et al., 2024
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/219233

Chitosan-based endodontic irrigation solutions and TGF-β1 treatment: Creating the most favourable environment for the survival and proliferation of stem cells of the apical papilla in vitro

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https://doi.org/10.1111/iej.14112

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Background: The dental pulp's environment is essential for the regulation of mesenchymal stem cells' homeostasis and thus, it is of great importance to evaluate the materials used in regenerative procedures. Aim: To assess in vitro (i) the effect of chitosan nanoparticles, 0.2% chitosan irrigation solution, Dual Rinse®, 17% EDTA, 10% citric acid and 2.5% NaOCl on DSCS viability; (ii) the effect of different concentrations of TGF-β1 on DCSC proliferation; and (iii) whether treatment with TGF-β1 following exposure to the different irrigation solutions could compensate for their negative effects. Methodology: (i) DSCS were treated with three dilutions (1:10, 1:100 and 1:1000) of the six irrigation solutions prepared in DMEM for 10 and 60min to assess the effect on viability. (ii) The effect of different concentrations (0, 1, 5 and 10ng/mL) of TGFβ1 on DCSC proliferation was assessed at 1, 3 and 7days. (iii) The proliferative effect of TGF-β1 following 10-min exposure to 1:10 dilution of each irrigation solution was also tested. We used MTT assay to assess viability and proliferation. We performed statistical analysis using Prism software. Results: (i) The different endodontic irrigation solutions tested showed a significant effect on cell viability (p≤.0001). Significant interactions between the endodontic irrigation solutions and their dilutions were also found for all parameters (p≤.0001). Chitosan nanoparticles and 0.2% chitosan irrigation solution were the least cytotoxic to DSCS whilst 2.5% NaOCl was the most cytotoxic followed by 17% EDTA. (ii) TGF-β1 at concentrations of 1 and 5ng/mL resulted in significantly higher proliferation compared to the control group. (iii) Exposure to 17% EDTA or 2.5% NaOCl for 10min was sufficient to make DSCS cells refractory to the proliferative effects of TGF-β1. DSCS groups treated with TGF-β1 following exposure to chitosan nanoparticles, 0.2% chitosan irrigation solution.

Matèries

Proliferació cel·lular, Quitosan, Polpa dental

Matèries (anglès)

Cell proliferation, Chitosan, Dental pulp

Citació

Col·leccions

Articles publicats en revistes (Odontoestomatologia)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citació

BELKADI, Roumaissa, et al. Chitosan-based endodontic irrigation solutions and TGF-β1 treatment: Creating the most favourable environment for the survival and proliferation of stem cells of the apical papilla in vitro. International Endodontic Journal. 2024. Vol. 57, num. 10, pags. 1942-1504. ISSN 0143-2885. [consulted: 3 of August of 2026]. Available at: https://hdl.handle.net/2445/219233

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