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2024-10-01

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cc by-nc-nd (c) Belkadi, Roumaissa et al., 2024
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/219233

Chitosan-based endodontic irrigation solutions and TGF-β1 treatment: Creating the most favourable environment for the survival and proliferation of stem cells of the apical papilla in vitro

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https://doi.org/10.1111/iej.14112

Abstract

Background: The dental pulp's environment is essential for the regulation of mesenchymal stem cells' homeostasis and thus, it is of great importance to evaluate the materials used in regenerative procedures. Aim: To assess in vitro (i) the effect of chitosan nanoparticles, 0.2% chitosan irrigation solution, Dual Rinse®, 17% EDTA, 10% citric acid and 2.5% NaOCl on DSCS viability; (ii) the effect of different concentrations of TGF-β1 on DCSC proliferation; and (iii) whether treatment with TGF-β1 following exposure to the different irrigation solutions could compensate for their negative effects. Methodology: (i) DSCS were treated with three dilutions (1:10, 1:100 and 1:1000) of the six irrigation solutions prepared in DMEM for 10 and 60min to assess the effect on viability. (ii) The effect of different concentrations (0, 1, 5 and 10ng/mL) of TGFβ1 on DCSC proliferation was assessed at 1, 3 and 7days. (iii) The proliferative effect of TGF-β1 following 10-min exposure to 1:10 dilution of each irrigation solution was also tested. We used MTT assay to assess viability and proliferation. We performed statistical analysis using Prism software. Results: (i) The different endodontic irrigation solutions tested showed a significant effect on cell viability (p≤.0001). Significant interactions between the endodontic irrigation solutions and their dilutions were also found for all parameters (p≤.0001). Chitosan nanoparticles and 0.2% chitosan irrigation solution were the least cytotoxic to DSCS whilst 2.5% NaOCl was the most cytotoxic followed by 17% EDTA. (ii) TGF-β1 at concentrations of 1 and 5ng/mL resulted in significantly higher proliferation compared to the control group. (iii) Exposure to 17% EDTA or 2.5% NaOCl for 10min was sufficient to make DSCS cells refractory to the proliferative effects of TGF-β1. DSCS groups treated with TGF-β1 following exposure to chitosan nanoparticles, 0.2% chitosan irrigation solution.

Subject

Proliferació cel·lular, Quitosan, Polpa dental

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Cell proliferation, Chitosan, Dental pulp

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Articles publicats en revistes (Odontoestomatologia)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Citation

BELKADI, Roumaissa, et al. Chitosan-based endodontic irrigation solutions and TGF-β1 treatment: Creating the most favourable environment for the survival and proliferation of stem cells of the apical papilla in vitro. International Endodontic Journal. 2024. Vol. 57, num. 10, pags. 1942-1504. ISSN 0143-2885. [consulted: 11 of August of 2026]. Available at: https://hdl.handle.net/2445/219233

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