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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/145441
Microbiota analysis of biofilms on experimental abutments mimicking dental implants: an in vivo model
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Background: the microbiota colonizing dental implants has been said to be similar to the microbiome surrounding teeth. In the absence of inflammation, a biofilm with pathologic bacteria can cover implant surfaces exposed to the oral cavity, for example, due to a remodeling process. The aim of the present study is to identify microbiota surrounding exposed dental implants in patients with and without a history of periodontitis through a deep-sequencing approach. Methods: an experimental abutment with the same surface and structure as a commercially available dental implant was used. Bacterial DNA was isolated, and the 16S ribosomal RNA gene was amplified and sequenced. Multiplexed tag-encoded sequencing of DNA from the samples was performed, and the reads were processed by metagenomic rapid annotation.Results: a wide variety of bacteria, 96 species, were identified. The most frequently found bacteria were Fusobacterium nucleatum and Prevotella denticola. Some species generally associated with periodontitis were found to a greater extent in patients without a history of periodontitis. Some bacteria that have never been described as part of the oral microbiome were identified in the present sample.Conclusions: analysis of data suggests that the bacteria surrounding exposed dental implants form a diverse microbiome regardless of the periodontal profile of patients. Further research is needed to clarify the role of these microorganisms in the oral environment.
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CORTÉS ACHA, Berta, et al. Microbiota analysis of biofilms on experimental abutments mimicking dental implants: an in vivo model. Journal of Periodontology. 2017. Vol. 88, num. 10, pags. 1090-1104. ISSN 0022-3492. [consulted: 9 of October of 2026]. Available at: https://hdl.handle.net/2445/145441